Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16458 | 5' | -58.2 | NC_004084.1 | + | 41907 | 0.66 | 0.584838 |
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Target: 5'- gACCGcggCGCgCGAUCGCcuGCuguGGCGAu -3' miRNA: 3'- gUGGCa--GUG-GCUAGCGcuCGu--CCGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 35710 | 0.66 | 0.584838 |
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Target: 5'- aGCCG-CACuCGAgguucucagggCGCGAGguGGCa- -3' miRNA: 3'- gUGGCaGUG-GCUa----------GCGCUCguCCGcu -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 13607 | 0.66 | 0.595467 |
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Target: 5'- aUACCGUCACCGA-C-CGGGUGGuCGGg -3' miRNA: 3'- -GUGGCAGUGGCUaGcGCUCGUCcGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 21581 | 0.66 | 0.606125 |
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Target: 5'- -uUCGUCACCGAUgcaaccgGCGAGCAGcUGGa -3' miRNA: 3'- guGGCAGUGGCUAg------CGCUCGUCcGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 31282 | 0.66 | 0.606125 |
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Target: 5'- --aCGUgGCCGGUCGCGGGUcccuaucguugGGGUa- -3' miRNA: 3'- gugGCAgUGGCUAGCGCUCG-----------UCCGcu -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 47960 | 0.66 | 0.606125 |
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Target: 5'- gGCCGUCcUgGAUCGCcucGCGGGCu- -3' miRNA: 3'- gUGGCAGuGgCUAGCGcu-CGUCCGcu -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 29770 | 0.66 | 0.606125 |
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Target: 5'- gACCGUC-CUGcAUCGCGA-CGGGuCGGc -3' miRNA: 3'- gUGGCAGuGGC-UAGCGCUcGUCC-GCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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