Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16458 | 5' | -58.2 | NC_004084.1 | + | 7391 | 0.67 | 0.542768 |
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Target: 5'- gACCG-CAUCGAcgaGCuGAGCAaGGCGAu -3' miRNA: 3'- gUGGCaGUGGCUag-CG-CUCGU-CCGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 7180 | 0.73 | 0.228345 |
|
Target: 5'- aGCCGccuaucagUACCGAgagugggaUCGCGAGCAGcGCGAg -3' miRNA: 3'- gUGGCa-------GUGGCU--------AGCGCUCGUC-CGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 5341 | 0.71 | 0.307501 |
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Target: 5'- --aCGUCGCCGA--GCGGGC-GGCGAg -3' miRNA: 3'- gugGCAGUGGCUagCGCUCGuCCGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 4520 | 0.68 | 0.442959 |
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Target: 5'- uCGCC-UCGCag--CGCGuGCAGGCGAu -3' miRNA: 3'- -GUGGcAGUGgcuaGCGCuCGUCCGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 3561 | 0.71 | 0.314968 |
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Target: 5'- gGCagGUCGguCCGAUggaaaGCGAGCGGGCGAc -3' miRNA: 3'- gUGg-CAGU--GGCUAg----CGCUCGUCCGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 396 | 0.74 | 0.211453 |
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Target: 5'- uCACC-UCACCGAgaGCGAGCAGGaGAu -3' miRNA: 3'- -GUGGcAGUGGCUagCGCUCGUCCgCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 43 | 0.71 | 0.33031 |
|
Target: 5'- cCGCCGUCgGCCGAUUucUGAGCcgGGGCGGg -3' miRNA: 3'- -GUGGCAG-UGGCUAGc-GCUCG--UCCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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