Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16458 | 5' | -58.2 | NC_004084.1 | + | 53226 | 0.67 | 0.501744 |
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Target: 5'- gCACCGUCGugUC-AUCuaCGAGCGGGCGGu -3' miRNA: 3'- -GUGGCAGU--GGcUAGc-GCUCGUCCGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 28644 | 0.68 | 0.491696 |
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Target: 5'- aCGCCGaC-CCG-UCGCGAuGCAGGaCGGu -3' miRNA: 3'- -GUGGCaGuGGCuAGCGCU-CGUCC-GCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 9095 | 0.68 | 0.471889 |
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Target: 5'- uCGCCGaUAUCGAgaguaGCGAGCAGGUc- -3' miRNA: 3'- -GUGGCaGUGGCUag---CGCUCGUCCGcu -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 49845 | 0.68 | 0.471889 |
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Target: 5'- uCACuCGUCGaCG-UCGCGAGCGagauccGGCGAg -3' miRNA: 3'- -GUG-GCAGUgGCuAGCGCUCGU------CCGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 41676 | 0.68 | 0.471889 |
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Target: 5'- --aCGUCAUCGAggcCGCGAuGCAgaccGGCGAg -3' miRNA: 3'- gugGCAGUGGCUa--GCGCU-CGU----CCGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 38370 | 0.68 | 0.462137 |
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Target: 5'- aCGCCGg-AUCGAgCGCGGGaAGGCGAc -3' miRNA: 3'- -GUGGCagUGGCUaGCGCUCgUCCGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 42722 | 1.08 | 0.000708 |
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Target: 5'- uCACCGUCACCGAUCGCGAGCAGGCGAa -3' miRNA: 3'- -GUGGCAGUGGCUAGCGCUCGUCCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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