Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16460 | 3' | -60.2 | NC_004084.1 | + | 23062 | 0.66 | 0.490772 |
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Target: 5'- uCGCGaucGUCGCCggcgacgCGAcGAGGUGGACGu -3' miRNA: 3'- -GUGCcu-CAGCGG-------GCUcCUCCACCUGC- -5' |
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| 16460 | 3' | -60.2 | NC_004084.1 | + | 57406 | 0.67 | 0.416453 |
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Target: 5'- uCGCGucGAcGUCGCCggCGAGcucGAGGUGGACa -3' miRNA: 3'- -GUGC--CU-CAGCGG--GCUC---CUCCACCUGc -5' |
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| 16460 | 3' | -60.2 | NC_004084.1 | + | 49036 | 0.67 | 0.416453 |
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Target: 5'- gAUGGGGaUGCCCGAGGAGGg----- -3' miRNA: 3'- gUGCCUCaGCGGGCUCCUCCaccugc -5' |
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| 16460 | 3' | -60.2 | NC_004084.1 | + | 41096 | 0.68 | 0.390081 |
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Target: 5'- uGCGGGccGUCGCCgcgggaGAGGAGGUuGAUGc -3' miRNA: 3'- gUGCCU--CAGCGGg-----CUCCUCCAcCUGC- -5' |
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| 16460 | 3' | -60.2 | NC_004084.1 | + | 44827 | 0.68 | 0.390081 |
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Target: 5'- gAUGGGGUCGCUCGAGuuGAGGUuGAa- -3' miRNA: 3'- gUGCCUCAGCGGGCUC--CUCCAcCUgc -5' |
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| 16460 | 3' | -60.2 | NC_004084.1 | + | 7511 | 0.7 | 0.303164 |
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Target: 5'- gACGGGGagacugCGCCCGAGuGGGUgcagccgauccaGGACGg -3' miRNA: 3'- gUGCCUCa-----GCGGGCUCcUCCA------------CCUGC- -5' |
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| 16460 | 3' | -60.2 | NC_004084.1 | + | 54558 | 0.7 | 0.296046 |
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Target: 5'- uCGCGGGGUUGgacaCCGAGGuuccGGUGGAg- -3' miRNA: 3'- -GUGCCUCAGCg---GGCUCCu---CCACCUgc -5' |
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| 16460 | 3' | -60.2 | NC_004084.1 | + | 6435 | 0.72 | 0.220606 |
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Target: 5'- gACGuGGUgCGCCCGAGGAGGgugcuGGCGg -3' miRNA: 3'- gUGCcUCA-GCGGGCUCCUCCac---CUGC- -5' |
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| 16460 | 3' | -60.2 | NC_004084.1 | + | 43178 | 1.08 | 0.000509 |
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Target: 5'- uCACGGAGUCGCCCGAGGAGGUGGACGa -3' miRNA: 3'- -GUGCCUCAGCGGGCUCCUCCACCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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