Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1647 | 3' | -59.9 | NC_001347.2 | + | 89738 | 0.69 | 0.698652 |
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Target: 5'- -cGC-CGUCGUCGCCUGCGgcGUcGGCg -3' miRNA: 3'- gcCGaGCAGCAGUGGGCGCa-CA-CCGg -5' |
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| 1647 | 3' | -59.9 | NC_001347.2 | + | 85344 | 0.7 | 0.670272 |
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Target: 5'- -cGCcagCGcCGUCGCCCGCGgcuaGGCCg -3' miRNA: 3'- gcCGa--GCaGCAGUGGGCGCaca-CCGG- -5' |
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| 1647 | 3' | -59.9 | NC_001347.2 | + | 75970 | 0.7 | 0.641648 |
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Target: 5'- gGGCUgCGgcgaGUCGCCCaGCGgGUcGGCCa -3' miRNA: 3'- gCCGA-GCag--CAGUGGG-CGCaCA-CCGG- -5' |
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| 1647 | 3' | -59.9 | NC_001347.2 | + | 39096 | 0.74 | 0.41477 |
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Target: 5'- uGGCUgCGcggCGUCACCCGCGcGUagaauccguacGGCCg -3' miRNA: 3'- gCCGA-GCa--GCAGUGGGCGCaCA-----------CCGG- -5' |
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| 1647 | 3' | -59.9 | NC_001347.2 | + | 194472 | 0.75 | 0.398665 |
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Target: 5'- aCGGCggGUCGUUggcuCCCGCuGUGcUGGCCg -3' miRNA: 3'- -GCCGagCAGCAGu---GGGCG-CAC-ACCGG- -5' |
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| 1647 | 3' | -59.9 | NC_001347.2 | + | 128324 | 1.12 | 0.001624 |
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Target: 5'- gCGGCUCGUCGUCACCCGCGUGUGGCCg -3' miRNA: 3'- -GCCGAGCAGCAGUGGGCGCACACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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