Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16470 | 3' | -59.5 | NC_004084.1 | + | 11510 | 0.69 | 0.33635 |
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Target: 5'- -aGGCAucugcaucaacaccuCCUGCAgccgCCGGgGCGugGCg -3' miRNA: 3'- agCCGU---------------GGACGUa---GGCCaCGCugCGg -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 17236 | 0.7 | 0.309707 |
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Target: 5'- cUCGGUgaagcagcaggAUCUGCggCCGGUgcagucgGCGAUGCCc -3' miRNA: 3'- -AGCCG-----------UGGACGuaGGCCA-------CGCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 21384 | 0.7 | 0.303122 |
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Target: 5'- gUCGGUGCCagcucggGCAugaUCCGGU-CGACGUCc -3' miRNA: 3'- -AGCCGUGGa------CGU---AGGCCAcGCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 17659 | 0.7 | 0.295931 |
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Target: 5'- uUCGGCuucgaCUGCcgaGUCCGGUGCu-UGCCc -3' miRNA: 3'- -AGCCGug---GACG---UAGGCCACGcuGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 29989 | 0.7 | 0.288874 |
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Target: 5'- cCGGCgucgACCUgGUcgCUGGUGaCGACGUCa -3' miRNA: 3'- aGCCG----UGGA-CGuaGGCCAC-GCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 29416 | 0.7 | 0.288874 |
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Target: 5'- -gGGCuCCUGCAUCgCGGUcaucagGCcGACGUCg -3' miRNA: 3'- agCCGuGGACGUAG-GCCA------CG-CUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 54658 | 0.71 | 0.254934 |
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Target: 5'- aCGGCACCUGUGucagcggguuUCCGGUcGCGAuugacuggcaaacCGCUc -3' miRNA: 3'- aGCCGUGGACGU----------AGGCCA-CGCU-------------GCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 34951 | 0.72 | 0.231243 |
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Target: 5'- uUCGuGCGCUaGCggCUGGUcGCGACGCUg -3' miRNA: 3'- -AGC-CGUGGaCGuaGGCCA-CGCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 47113 | 1.12 | 0.000267 |
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Target: 5'- gUCGGCACCUGCAUCCGGUGCGACGCCg -3' miRNA: 3'- -AGCCGUGGACGUAGGCCACGCUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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