Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16470 | 3' | -59.5 | NC_004084.1 | + | 22458 | 0.67 | 0.436095 |
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Target: 5'- gUCGGgACUggucacgGCcgCCGaccccgGCGACGCCg -3' miRNA: 3'- -AGCCgUGGa------CGuaGGCca----CGCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 21384 | 0.7 | 0.303122 |
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Target: 5'- gUCGGUGCCagcucggGCAugaUCCGGU-CGACGUCc -3' miRNA: 3'- -AGCCGUGGa------CGU---AGGCCAcGCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 18572 | 0.66 | 0.493928 |
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Target: 5'- aCGGCGCCgcgGCcgCCGaUGgacuCGAgCGCCa -3' miRNA: 3'- aGCCGUGGa--CGuaGGCcAC----GCU-GCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 17659 | 0.7 | 0.295931 |
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Target: 5'- uUCGGCuucgaCUGCcgaGUCCGGUGCu-UGCCc -3' miRNA: 3'- -AGCCGug---GACG---UAGGCCACGcuGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 17236 | 0.7 | 0.309707 |
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Target: 5'- cUCGGUgaagcagcaggAUCUGCggCCGGUgcagucgGCGAUGCCc -3' miRNA: 3'- -AGCCG-----------UGGACGuaGGCCA-------CGCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 16937 | 0.67 | 0.454951 |
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Target: 5'- uUCGGCAUCgGCGggCGucGCGACGUCg -3' miRNA: 3'- -AGCCGUGGaCGUagGCcaCGCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 16886 | 0.66 | 0.524134 |
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Target: 5'- gCGGCGUCUGC-UCCGau-CGACGCUg -3' miRNA: 3'- aGCCGUGGACGuAGGCcacGCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 14491 | 0.66 | 0.524134 |
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Target: 5'- gUCGGCuCCUGCcucggccaacUCgGGUuCGugGCCc -3' miRNA: 3'- -AGCCGuGGACGu---------AGgCCAcGCugCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 11510 | 0.69 | 0.33635 |
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Target: 5'- -aGGCAucugcaucaacaccuCCUGCAgccgCCGGgGCGugGCg -3' miRNA: 3'- agCCGU---------------GGACGUa---GGCCaCGCugCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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