Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16470 | 3' | -59.5 | NC_004084.1 | + | 16937 | 0.67 | 0.454951 |
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Target: 5'- uUCGGCAUCgGCGggCGucGCGACGUCg -3' miRNA: 3'- -AGCCGUGGaCGUagGCcaCGCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 48910 | 0.67 | 0.464543 |
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Target: 5'- gUCGGUcgGCCacgacuUCCGGU-CGGCGCCg -3' miRNA: 3'- -AGCCG--UGGacgu--AGGCCAcGCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 18572 | 0.66 | 0.493928 |
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Target: 5'- aCGGCGCCgcgGCcgCCGaUGgacuCGAgCGCCa -3' miRNA: 3'- aGCCGUGGa--CGuaGGCcAC----GCU-GCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 49513 | 0.66 | 0.493928 |
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Target: 5'- uUCGGCuuccGCCUcuuCGUCCGaGU-CGACGCUg -3' miRNA: 3'- -AGCCG----UGGAc--GUAGGC-CAcGCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 54565 | 0.66 | 0.493928 |
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Target: 5'- gUUGGaCACCgaGguUCCGGUGgagccgucguCGGCGUCg -3' miRNA: 3'- -AGCC-GUGGa-CguAGGCCAC----------GCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 40847 | 0.66 | 0.503912 |
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Target: 5'- ---cCGCCUGC-UCUGGUGCGucccACGCUc -3' miRNA: 3'- agccGUGGACGuAGGCCACGC----UGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 22942 | 0.66 | 0.513983 |
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Target: 5'- cUCGGCgaucgccccucgACCUGC-UCCuGGU-CGAUGUCa -3' miRNA: 3'- -AGCCG------------UGGACGuAGG-CCAcGCUGCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 14491 | 0.66 | 0.524134 |
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Target: 5'- gUCGGCuCCUGCcucggccaacUCgGGUuCGugGCCc -3' miRNA: 3'- -AGCCGuGGACGu---------AGgCCAcGCugCGG- -5' |
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| 16470 | 3' | -59.5 | NC_004084.1 | + | 29989 | 0.7 | 0.288874 |
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Target: 5'- cCGGCgucgACCUgGUcgCUGGUGaCGACGUCa -3' miRNA: 3'- aGCCG----UGGA-CGuaGGCCAC-GCUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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