Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16470 | 3' | -59.5 | NC_004084.1 | + | 43839 | 0.68 | 0.426837 |
Target: 5'- -gGGCGCgaGaCAUCC---GCGACGCCg -3' miRNA: 3'- agCCGUGgaC-GUAGGccaCGCUGCGG- -5' |
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16470 | 3' | -59.5 | NC_004084.1 | + | 22458 | 0.67 | 0.436095 |
Target: 5'- gUCGGgACUggucacgGCcgCCGaccccgGCGACGCCg -3' miRNA: 3'- -AGCCgUGGa------CGuaGGCca----CGCUGCGG- -5' |
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16470 | 3' | -59.5 | NC_004084.1 | + | 51463 | 0.67 | 0.445467 |
Target: 5'- gCGGCGgCUGUA--CGG-GCGACGaCCg -3' miRNA: 3'- aGCCGUgGACGUagGCCaCGCUGC-GG- -5' |
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16470 | 3' | -59.5 | NC_004084.1 | + | 55153 | 0.66 | 0.493928 |
Target: 5'- aCGGCACgaGCGUuggCCGGguucGCGACaGUUa -3' miRNA: 3'- aGCCGUGgaCGUA---GGCCa---CGCUG-CGG- -5' |
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16470 | 3' | -59.5 | NC_004084.1 | + | 43782 | 0.66 | 0.513983 |
Target: 5'- -gGGCGCUcGCcggcggagaGUCCGGcgGCGaggaGCGCCg -3' miRNA: 3'- agCCGUGGaCG---------UAGGCCa-CGC----UGCGG- -5' |
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16470 | 3' | -59.5 | NC_004084.1 | + | 16886 | 0.66 | 0.524134 |
Target: 5'- gCGGCGUCUGC-UCCGau-CGACGCUg -3' miRNA: 3'- aGCCGUGGACGuAGGCcacGCUGCGG- -5' |
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16470 | 3' | -59.5 | NC_004084.1 | + | 46571 | 0.66 | 0.524134 |
Target: 5'- cUCGGCAUCgGCAUCgCG--GCGAuCGUCg -3' miRNA: 3'- -AGCCGUGGaCGUAG-GCcaCGCU-GCGG- -5' |
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16470 | 3' | -59.5 | NC_004084.1 | + | 27528 | 0.66 | 0.534362 |
Target: 5'- aCGGCuACCUGgAUggaCCGGUgGCcGCGCa -3' miRNA: 3'- aGCCG-UGGACgUA---GGCCA-CGcUGCGg -5' |
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16470 | 3' | -59.5 | NC_004084.1 | + | 47113 | 1.12 | 0.000267 |
Target: 5'- gUCGGCACCUGCAUCCGGUGCGACGCCg -3' miRNA: 3'- -AGCCGUGGACGUAGGCCACGCUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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