Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
16478 | 5' | -55.6 | NC_004084.1 | + | 8574 | 0.77 | 0.188952 |
Target: 5'- cUCGCUCG-CGACGUCGaCGAGUGAuucGAUg -3' miRNA: 3'- -AGUGAGCaGCUGCAGC-GCUCGCU---CUA- -5' |
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16478 | 5' | -55.6 | NC_004084.1 | + | 30935 | 0.77 | 0.179132 |
Target: 5'- aUCGCUCGUCGACGUCGaGcuCGAGGa -3' miRNA: 3'- -AGUGAGCAGCUGCAGCgCucGCUCUa -5' |
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16478 | 5' | -55.6 | NC_004084.1 | + | 22985 | 0.8 | 0.122293 |
Target: 5'- gUC-CUgGUCGACcUCGCGAGCGAGAUc -3' miRNA: 3'- -AGuGAgCAGCUGcAGCGCUCGCUCUA- -5' |
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16478 | 5' | -55.6 | NC_004084.1 | + | 49844 | 1.06 | 0.001839 |
Target: 5'- aUCACUCGUCGACGUCGCGAGCGAGAUc -3' miRNA: 3'- -AGUGAGCAGCUGCAGCGCUCGCUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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