miRNA: 3'- -CgAUACAGCGCCUG-----AugCUGCGCAGa -5'
miRNA: 3'- cGAUACaGCGcCUG-AUGCUGCGCAGa -5'
miRNA: 3'- cGAUACAG---------------CGCCUGAUG-CUGCGCaGA- -5'
miRNA: 3'- -CGAUACAGCGCCUGAUGCUGCGCAGA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1648 | 5' | -53.1 | NC_001347.2 | + | 78 | 0.7 | 0.931639 |
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Target: 5'- gGgUGUGUCGCGGGCgugugUugGGUGUGUCg -3' miRNA: 3'- -CgAUACAGCGCCUG-----AugCUGCGCAGa -5' |
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| 1648 | 5' | -53.1 | NC_001347.2 | + | 3171 | 0.73 | 0.847476 |
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Target: 5'- aUUGUGcCGC-GACaUGCGACGCGUCa -3' miRNA: 3'- cGAUACaGCGcCUG-AUGCUGCGCAGa -5' |
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| 1648 | 5' | -53.1 | NC_001347.2 | + | 114456 | 0.73 | 0.826471 |
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Target: 5'- cCUGUGUCacccgcgcuugagcgGCGGACUGCuGACGCG-CUu -3' miRNA: 3'- cGAUACAG---------------CGCCUGAUG-CUGCGCaGA- -5' |
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| 1648 | 5' | -53.1 | NC_001347.2 | + | 127463 | 1.11 | 0.007284 |
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Target: 5'- aGCUAUGUCGCGGACUACGACGCGUCUg -3' miRNA: 3'- -CGAUACAGCGCCUGAUGCUGCGCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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