Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
16481 | 3' | -54.4 | NC_004084.1 | + | 49118 | 0.67 | 0.731839 |
Target: 5'- gGCUCGAGUGugGuguugauCCACUU-CUCGAc -3' miRNA: 3'- aUGGGCUCAUugCu------GGUGAGcGAGCU- -5' |
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16481 | 3' | -54.4 | NC_004084.1 | + | 8663 | 0.66 | 0.763049 |
Target: 5'- --gCCGAGggucugGGCGAcCCACUCGagcacCUCGAa -3' miRNA: 3'- augGGCUCa-----UUGCU-GGUGAGC-----GAGCU- -5' |
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16481 | 3' | -54.4 | NC_004084.1 | + | 19508 | 0.66 | 0.773199 |
Target: 5'- aGCCCGGG-AGCGaacGCCGgUUcgaGCUCGAa -3' miRNA: 3'- aUGGGCUCaUUGC---UGGUgAG---CGAGCU- -5' |
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16481 | 3' | -54.4 | NC_004084.1 | + | 26267 | 0.66 | 0.783202 |
Target: 5'- cGCCCGuGUcccaGAUGguGCCGCUCGCgaggucugUCGAc -3' miRNA: 3'- aUGGGCuCA----UUGC--UGGUGAGCG--------AGCU- -5' |
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16481 | 3' | -54.4 | NC_004084.1 | + | 40791 | 0.66 | 0.793048 |
Target: 5'- aACUCGgcAGUAACGAgCUGCUCGCgaagcuggCGAu -3' miRNA: 3'- aUGGGC--UCAUUGCU-GGUGAGCGa-------GCU- -5' |
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16481 | 3' | -54.4 | NC_004084.1 | + | 1705 | 0.65 | 0.801766 |
Target: 5'- -gUCCGAGUcccagcggaggauGGCGGCUGCUCGaUCGGu -3' miRNA: 3'- auGGGCUCA-------------UUGCUGGUGAGCgAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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