Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16481 | 3' | -54.4 | NC_004084.1 | + | 8663 | 0.66 | 0.763049 |
Target: 5'- --gCCGAGggucugGGCGAcCCACUCGagcacCUCGAa -3' miRNA: 3'- augGGCUCa-----UUGCU-GGUGAGC-----GAGCU- -5' |
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16481 | 3' | -54.4 | NC_004084.1 | + | 6378 | 0.69 | 0.601006 |
Target: 5'- -uCCCGAcgccguaGACCGCUCGCUCa- -3' miRNA: 3'- auGGGCUcauug--CUGGUGAGCGAGcu -5' |
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16481 | 3' | -54.4 | NC_004084.1 | + | 5162 | 0.73 | 0.360947 |
Target: 5'- gGCCCGAucucgucgccGUcGACGACCGCcCGCUCGu -3' miRNA: 3'- aUGGGCU----------CA-UUGCUGGUGaGCGAGCu -5' |
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16481 | 3' | -54.4 | NC_004084.1 | + | 2079 | 0.68 | 0.677973 |
Target: 5'- aGCCuCGAG-AACGGCauCGCUCGCcugCGAa -3' miRNA: 3'- aUGG-GCUCaUUGCUG--GUGAGCGa--GCU- -5' |
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16481 | 3' | -54.4 | NC_004084.1 | + | 1705 | 0.65 | 0.801766 |
Target: 5'- -gUCCGAGUcccagcggaggauGGCGGCUGCUCGaUCGGu -3' miRNA: 3'- auGGGCUCA-------------UUGCUGGUGAGCgAGCU- -5' |
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16481 | 3' | -54.4 | NC_004084.1 | + | 1281 | 0.76 | 0.261667 |
Target: 5'- cGCCCGGG-GAUGcCCgGCUCGCUCGAu -3' miRNA: 3'- aUGGGCUCaUUGCuGG-UGAGCGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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