Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16481 | 3' | -54.4 | NC_004084.1 | + | 2079 | 0.68 | 0.677973 |
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Target: 5'- aGCCuCGAG-AACGGCauCGCUCGCcugCGAa -3' miRNA: 3'- aUGG-GCUCaUUGCUG--GUGAGCGa--GCU- -5' |
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| 16481 | 3' | -54.4 | NC_004084.1 | + | 29055 | 0.68 | 0.688878 |
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Target: 5'- cUGCUgGcAGgccGCGACCGCcCGCUCGGg -3' miRNA: 3'- -AUGGgC-UCau-UGCUGGUGaGCGAGCU- -5' |
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| 16481 | 3' | -54.4 | NC_004084.1 | + | 55284 | 0.68 | 0.688878 |
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Target: 5'- --gCUGGGUGuCGAUCGCUUGCUCa- -3' miRNA: 3'- augGGCUCAUuGCUGGUGAGCGAGcu -5' |
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| 16481 | 3' | -54.4 | NC_004084.1 | + | 39706 | 0.67 | 0.699728 |
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Target: 5'- aACCCGccAGUGACGGCgACgaCGCcgUCGAc -3' miRNA: 3'- aUGGGC--UCAUUGCUGgUGa-GCG--AGCU- -5' |
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| 16481 | 3' | -54.4 | NC_004084.1 | + | 24344 | 0.67 | 0.72122 |
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Target: 5'- gAUCUGAGcaacGCGACCA--CGCUCGAg -3' miRNA: 3'- aUGGGCUCau--UGCUGGUgaGCGAGCU- -5' |
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| 16481 | 3' | -54.4 | NC_004084.1 | + | 50296 | 1.07 | 0.001755 |
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Target: 5'- cUACCCGAGUAACGACCACUCGCUCGAg -3' miRNA: 3'- -AUGGGCUCAUUGCUGGUGAGCGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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