Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
16485 | 3' | -57.6 | NC_004084.1 | + | 51838 | 1.07 | 0.000813 |
Target: 5'- gUCGCCACUCUCGAAGAGCGCGUGGCUc -3' miRNA: 3'- -AGCGGUGAGAGCUUCUCGCGCACCGA- -5' |
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16485 | 3' | -57.6 | NC_004084.1 | + | 21015 | 0.73 | 0.254556 |
Target: 5'- -gGCCAUcCUCGA--GGCGCGUGGUg -3' miRNA: 3'- agCGGUGaGAGCUucUCGCGCACCGa -5' |
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16485 | 3' | -57.6 | NC_004084.1 | + | 35710 | 0.71 | 0.341012 |
Target: 5'- -aGCCGCaCUCGAgguucucAGGGCGCGaggUGGCa -3' miRNA: 3'- agCGGUGaGAGCU-------UCUCGCGC---ACCGa -5' |
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16485 | 3' | -57.6 | NC_004084.1 | + | 25332 | 0.7 | 0.349997 |
Target: 5'- gCGCCcCuUCUCGcAGGGCuCGUGGCUc -3' miRNA: 3'- aGCGGuG-AGAGCuUCUCGcGCACCGA- -5' |
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16485 | 3' | -57.6 | NC_004084.1 | + | 37592 | 0.67 | 0.51899 |
Target: 5'- -aGgCACUCUCGggGAGCGCu----- -3' miRNA: 3'- agCgGUGAGAGCuuCUCGCGcaccga -5' |
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16485 | 3' | -57.6 | NC_004084.1 | + | 53951 | 0.67 | 0.523143 |
Target: 5'- uUCGCCAg-CUCGAAcucgaucguccagucGGGCGCGUcgaGGCc -3' miRNA: 3'- -AGCGGUgaGAGCUU---------------CUCGCGCA---CCGa -5' |
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16485 | 3' | -57.6 | NC_004084.1 | + | 29191 | 0.67 | 0.536724 |
Target: 5'- cUCGCC-CUgCUCGGcgucgauguuccAGAGCGCGUcgacguuguucgcgGGCg -3' miRNA: 3'- -AGCGGuGA-GAGCU------------UCUCGCGCA--------------CCGa -5' |
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16485 | 3' | -57.6 | NC_004084.1 | + | 31623 | 0.67 | 0.539876 |
Target: 5'- cUCGauuCGCUCUgGGAGAGCGaCGUuGCg -3' miRNA: 3'- -AGCg--GUGAGAgCUUCUCGC-GCAcCGa -5' |
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16485 | 3' | -57.6 | NC_004084.1 | + | 51186 | 0.67 | 0.550427 |
Target: 5'- gCGCCGaUCgUCGAGGucggcgcuGGCGCG-GGCUa -3' miRNA: 3'- aGCGGUgAG-AGCUUC--------UCGCGCaCCGA- -5' |
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16485 | 3' | -57.6 | NC_004084.1 | + | 47569 | 0.66 | 0.61478 |
Target: 5'- aCGCCG--UUCGAGgacguucgcGAGCGCgGUGGCg -3' miRNA: 3'- aGCGGUgaGAGCUU---------CUCGCG-CACCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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