Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1649 | 3' | -56 | NC_001347.2 | + | 21438 | 0.71 | 0.739804 |
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Target: 5'- gCCGAGCUAugcGAGCGGGgacgGGACgACGa-- -3' miRNA: 3'- -GGCUCGAU---CUCGUCCa---CCUG-UGCguc -5' |
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| 1649 | 3' | -56 | NC_001347.2 | + | 151258 | 0.73 | 0.650958 |
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Target: 5'- -gGGGCUcGAacgaacgGCGGGUGGAgGCGCGGg -3' miRNA: 3'- ggCUCGAuCU-------CGUCCACCUgUGCGUC- -5' |
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| 1649 | 3' | -56 | NC_001347.2 | + | 198365 | 0.73 | 0.642009 |
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Target: 5'- aUGAGUuucUGGcGCAGGUGGgugGCGCGCGGg -3' miRNA: 3'- gGCUCG---AUCuCGUCCACC---UGUGCGUC- -5' |
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| 1649 | 3' | -56 | NC_001347.2 | + | 36680 | 0.75 | 0.5628 |
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Target: 5'- aCCGGGCUccGGCGGGUGGccugaGCACGCc- -3' miRNA: 3'- -GGCUCGAucUCGUCCACC-----UGUGCGuc -5' |
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| 1649 | 3' | -56 | NC_001347.2 | + | 216956 | 0.75 | 0.525043 |
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Target: 5'- cCCGAGCUGGAGCAGaacaauuccaaaccGGAUACGCu- -3' miRNA: 3'- -GGCUCGAUCUCGUCca------------CCUGUGCGuc -5' |
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| 1649 | 3' | -56 | NC_001347.2 | + | 126545 | 1.11 | 0.003227 |
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Target: 5'- aCCGAGCUAGAGCAGGUGGACACGCAGa -3' miRNA: 3'- -GGCUCGAUCUCGUCCACCUGUGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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