Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
1649 | 5' | -59.2 | NC_001347.2 | + | 65369 | 0.7 | 0.657798 |
Target: 5'- -aCCGCGcc---GUGCGUCUGCCCGu -3' miRNA: 3'- aaGGCGCuugcaCGCGCAGACGGGCu -5' |
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1649 | 5' | -59.2 | NC_001347.2 | + | 81459 | 0.7 | 0.63825 |
Target: 5'- -gUCGCGAACGUGCacggugaaagugGCGUCgucGCUCGGc -3' miRNA: 3'- aaGGCGCUUGCACG------------CGCAGa--CGGGCU- -5' |
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1649 | 5' | -59.2 | NC_001347.2 | + | 74934 | 0.71 | 0.608912 |
Target: 5'- -cCCuCGAGCGUGUaCGUCUGCgCCGGc -3' miRNA: 3'- aaGGcGCUUGCACGcGCAGACG-GGCU- -5' |
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1649 | 5' | -59.2 | NC_001347.2 | + | 126580 | 1.06 | 0.003708 |
Target: 5'- gUUCCGCGAACGUGCGCGUCUGCCCGAc -3' miRNA: 3'- -AAGGCGCUUGCACGCGCAGACGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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