Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16492 | 5' | -42 | NC_004084.1 | + | 29863 | 0.68 | 0.999872 |
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Target: 5'- aCCUauuccc---GAUGGGGUCGUGACg -3' miRNA: 3'- -GGAgauuacaaaCUAUUCCAGUACUG- -5' |
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| 16492 | 5' | -42 | NC_004084.1 | + | 10330 | 0.69 | 0.999671 |
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Target: 5'- gCCUCUGguucgacagcAUGUcgGAggagAAGGUCGUucGACg -3' miRNA: 3'- -GGAGAU----------UACAaaCUa---UUCCAGUA--CUG- -5' |
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| 16492 | 5' | -42 | NC_004084.1 | + | 4882 | 0.69 | 0.9993 |
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Target: 5'- ----aGAUGUUUGAUAAGGUgA-GGCg -3' miRNA: 3'- ggagaUUACAAACUAUUCCAgUaCUG- -5' |
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| 16492 | 5' | -42 | NC_004084.1 | + | 31807 | 0.7 | 0.998288 |
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Target: 5'- ----gAGUGUUUGAUAAGGUCugccgagcgucUGACg -3' miRNA: 3'- ggagaUUACAAACUAUUCCAGu----------ACUG- -5' |
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| 16492 | 5' | -42 | NC_004084.1 | + | 20800 | 0.71 | 0.995505 |
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Target: 5'- uUUCggugugcGUGUUUGAUAAGGUCuUGAg -3' miRNA: 3'- gGAGau-----UACAAACUAUUCCAGuACUg -5' |
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| 16492 | 5' | -42 | NC_004084.1 | + | 33576 | 0.75 | 0.958584 |
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Target: 5'- -gUCUAAuaaaucUGUUUGAUAAGGUCguagugaGUGGCg -3' miRNA: 3'- ggAGAUU------ACAAACUAUUCCAG-------UACUG- -5' |
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| 16492 | 5' | -42 | NC_004084.1 | + | 24871 | 0.86 | 0.528718 |
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Target: 5'- gCUCUAAacGUUUGAUAAGGUCAUGcCa -3' miRNA: 3'- gGAGAUUa-CAAACUAUUCCAGUACuG- -5' |
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| 16492 | 5' | -42 | NC_004084.1 | + | 37647 | 0.91 | 0.292749 |
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Target: 5'- cCCUUUGAUGUUUGAUAAGGUCuauucacgauaccaaAUGACu -3' miRNA: 3'- -GGAGAUUACAAACUAUUCCAG---------------UACUG- -5' |
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| 16492 | 5' | -42 | NC_004084.1 | + | 53574 | 1.15 | 0.013119 |
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Target: 5'- cCCUCUAAUGUUUGAUAAGGUCAUGACu -3' miRNA: 3'- -GGAGAUUACAAACUAUUCCAGUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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