Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16494 | 3' | -57.4 | NC_004084.1 | + | 55261 | 0.66 | 0.6025 |
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Target: 5'- gACGUCUacGGUcGGCGG-CGuGUAGAUCGu -3' miRNA: 3'- -UGUAGG--UCGaCCGCCaGC-CGUCUAGU- -5' |
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| 16494 | 3' | -57.4 | NC_004084.1 | + | 18825 | 0.66 | 0.591624 |
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Target: 5'- cACGUCCAGgaGGaCGGUgGcGCGGcuuUCGu -3' miRNA: 3'- -UGUAGGUCgaCC-GCCAgC-CGUCu--AGU- -5' |
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| 16494 | 3' | -57.4 | NC_004084.1 | + | 1206 | 0.66 | 0.591624 |
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Target: 5'- -gAUCCugaacGCUGGCuGGcUCGGCggcgGGAUCGa -3' miRNA: 3'- ugUAGGu----CGACCG-CC-AGCCG----UCUAGU- -5' |
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| 16494 | 3' | -57.4 | NC_004084.1 | + | 19001 | 0.66 | 0.569981 |
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Target: 5'- -gGUCCcGCUGGCGGggugggauccCGGCGuGUCGg -3' miRNA: 3'- ugUAGGuCGACCGCCa---------GCCGUcUAGU- -5' |
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| 16494 | 3' | -57.4 | NC_004084.1 | + | 29752 | 0.67 | 0.53791 |
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Target: 5'- gACGUUgAGCUcgcuGCGG-CGGUAGGUCGu -3' miRNA: 3'- -UGUAGgUCGAc---CGCCaGCCGUCUAGU- -5' |
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| 16494 | 3' | -57.4 | NC_004084.1 | + | 46184 | 0.7 | 0.381237 |
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Target: 5'- ---cCCAGCUGGUaGG-CGGCgAGAUCGu -3' miRNA: 3'- uguaGGUCGACCG-CCaGCCG-UCUAGU- -5' |
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| 16494 | 3' | -57.4 | NC_004084.1 | + | 25092 | 0.7 | 0.363871 |
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Target: 5'- uGCAUCCuucacGC-GGCGG-CGGCAGGUg- -3' miRNA: 3'- -UGUAGGu----CGaCCGCCaGCCGUCUAgu -5' |
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| 16494 | 3' | -57.4 | NC_004084.1 | + | 4064 | 0.7 | 0.347061 |
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Target: 5'- gACcgCCAGCUGGauGUCGGUGGuuuguUCGu -3' miRNA: 3'- -UGuaGGUCGACCgcCAGCCGUCu----AGU- -5' |
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| 16494 | 3' | -57.4 | NC_004084.1 | + | 53311 | 0.71 | 0.307505 |
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Target: 5'- gACGgucggCCAGCUGGCGGcccgccUgGGCAGAccgcUCAc -3' miRNA: 3'- -UGUa----GGUCGACCGCC------AgCCGUCU----AGU- -5' |
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| 16494 | 3' | -57.4 | NC_004084.1 | + | 54360 | 1.08 | 0.000719 |
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Target: 5'- gACAUCCAGCUGGCGGUCGGCAGAUCAc -3' miRNA: 3'- -UGUAGGUCGACCGCCAGCCGUCUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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