Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16496 | 3' | -47.5 | NC_004084.1 | + | 1451 | 0.66 | 0.986226 |
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Target: 5'- cGGCGauccUGGACUacuGGGA-CCAGCa---- -3' miRNA: 3'- -CCGCa---ACCUGA---UCCUaGGUUGauuuu -5' |
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| 16496 | 3' | -47.5 | NC_004084.1 | + | 5353 | 0.67 | 0.977015 |
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Target: 5'- gGGCGgcgaGGACUGGGAcgaUCuCGACg---- -3' miRNA: 3'- -CCGCaa--CCUGAUCCU---AG-GUUGauuuu -5' |
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| 16496 | 3' | -47.5 | NC_004084.1 | + | 18331 | 0.67 | 0.970996 |
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Target: 5'- uGGCG-UGGGCguuGGAUUCGACc---- -3' miRNA: 3'- -CCGCaACCUGau-CCUAGGUUGauuuu -5' |
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| 16496 | 3' | -47.5 | NC_004084.1 | + | 42624 | 0.68 | 0.955722 |
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Target: 5'- cGGCGUUGucaacGACUGGGAguUCCAGu----- -3' miRNA: 3'- -CCGCAAC-----CUGAUCCU--AGGUUgauuuu -5' |
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| 16496 | 3' | -47.5 | NC_004084.1 | + | 33450 | 0.74 | 0.73184 |
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Target: 5'- aGGCGaugaUGGGCUGGGcgaagcuGUCCAcaGCUGAGAa -3' miRNA: 3'- -CCGCa---ACCUGAUCC-------UAGGU--UGAUUUU- -5' |
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| 16496 | 3' | -47.5 | NC_004084.1 | + | 54717 | 1.1 | 0.00571 |
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Target: 5'- gGGCGUUGGACUAGGAUCCAACUAAAAg -3' miRNA: 3'- -CCGCAACCUGAUCCUAGGUUGAUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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