Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16498 | 3' | -55 | NC_004084.1 | + | 38632 | 0.66 | 0.714178 |
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Target: 5'- gCGgcUGAAGCC-GGUGGUCAgcagugagcacCGUCUGu -3' miRNA: 3'- aGC--ACUUCGGuCCGCCAGU-----------GCAGAUg -5' |
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| 16498 | 3' | -55 | NC_004084.1 | + | 27725 | 0.67 | 0.703434 |
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Target: 5'- -gGUGAggAGCCAGGCGaGacgCugGUCa-- -3' miRNA: 3'- agCACU--UCGGUCCGC-Ca--GugCAGaug -5' |
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| 16498 | 3' | -55 | NC_004084.1 | + | 21044 | 0.67 | 0.659873 |
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Target: 5'- uUCGUcGAAGUCAGGCGGcCACcgcagUGCu -3' miRNA: 3'- -AGCA-CUUCGGUCCGCCaGUGcag--AUG- -5' |
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| 16498 | 3' | -55 | NC_004084.1 | + | 13845 | 0.68 | 0.593953 |
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Target: 5'- gCGUGGcAGCCAGGCccGUCAuCGUUgagGCg -3' miRNA: 3'- aGCACU-UCGGUCCGc-CAGU-GCAGa--UG- -5' |
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| 16498 | 3' | -55 | NC_004084.1 | + | 16132 | 0.71 | 0.467145 |
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Target: 5'- aUCGUGAAcggguGaCCGcGGUGGUCGCG-CUGCu -3' miRNA: 3'- -AGCACUU-----C-GGU-CCGCCAGUGCaGAUG- -5' |
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| 16498 | 3' | -55 | NC_004084.1 | + | 49666 | 0.76 | 0.220489 |
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Target: 5'- cCGgcUGAAGCCGGGUGGUC-CGUCg-- -3' miRNA: 3'- aGC--ACUUCGGUCCGCCAGuGCAGaug -5' |
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| 16498 | 3' | -55 | NC_004084.1 | + | 56507 | 1.11 | 0.000848 |
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Target: 5'- gUCGUGAAGCCAGGCGGUCACGUCUACg -3' miRNA: 3'- -AGCACUUCGGUCCGCCAGUGCAGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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