Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
1650 | 3' | -54.6 | NC_001347.2 | + | 87163 | 0.71 | 0.790772 |
Target: 5'- cGG-GGAaacGcGCGUGCgggucgcagaugaGCAGCUUCUGCa -3' miRNA: 3'- aCCuCCU---CaUGCACG-------------CGUCGAAGACG- -5' |
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1650 | 3' | -54.6 | NC_001347.2 | + | 157992 | 0.72 | 0.754669 |
Target: 5'- cGGAGGAGgcucgACG-GCgGCAGCUgcgGCg -3' miRNA: 3'- aCCUCCUCa----UGCaCG-CGUCGAagaCG- -5' |
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1650 | 3' | -54.6 | NC_001347.2 | + | 126383 | 1.13 | 0.003206 |
Target: 5'- gUGGAGGAGUACGUGCGCAGCUUCUGCa -3' miRNA: 3'- -ACCUCCUCAUGCACGCGUCGAAGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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