Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16504 | 5' | -55.9 | NC_004084.1 | + | 41638 | 0.66 | 0.684023 |
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Target: 5'- cGC-CGCGGCgaACGGUg-CGAgGAGcGCCAu -3' miRNA: 3'- -CGaGCGCCG--UGUCAaaGCU-CUC-CGGU- -5' |
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| 16504 | 5' | -55.9 | NC_004084.1 | + | 47583 | 0.66 | 0.666635 |
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Target: 5'- cGUUCGCGaGCGCGGUggCGAcucucacaagaucucGAGGUg- -3' miRNA: 3'- -CGAGCGC-CGUGUCAaaGCU---------------CUCCGgu -5' |
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| 16504 | 5' | -55.9 | NC_004084.1 | + | 53146 | 0.67 | 0.629465 |
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Target: 5'- cGCU-GCGGgACGucgUCGAcGAGGCCGa -3' miRNA: 3'- -CGAgCGCCgUGUcaaAGCU-CUCCGGU- -5' |
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| 16504 | 5' | -55.9 | NC_004084.1 | + | 32670 | 0.69 | 0.521581 |
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Target: 5'- aGCUUGCGGCAauc-UUCGAGGcGGCgAa -3' miRNA: 3'- -CGAGCGCCGUgucaAAGCUCU-CCGgU- -5' |
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| 16504 | 5' | -55.9 | NC_004084.1 | + | 20300 | 0.69 | 0.499728 |
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Target: 5'- aGCUCGgGGCaACAGgccaucaUCGAcauacugacacagGAGGCCGc -3' miRNA: 3'- -CGAGCgCCG-UGUCaa-----AGCU-------------CUCCGGU- -5' |
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| 16504 | 5' | -55.9 | NC_004084.1 | + | 5251 | 0.69 | 0.480314 |
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Target: 5'- gGCuUCGCGGCGCAca--CGAGgaGGGCCu -3' miRNA: 3'- -CG-AGCGCCGUGUcaaaGCUC--UCCGGu -5' |
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| 16504 | 5' | -55.9 | NC_004084.1 | + | 49934 | 0.7 | 0.450435 |
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Target: 5'- ---gGCGGCGCAGguggUCaGuGGGGCCAg -3' miRNA: 3'- cgagCGCCGUGUCaa--AG-CuCUCCGGU- -5' |
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| 16504 | 5' | -55.9 | NC_004084.1 | + | 58056 | 1.1 | 0.000785 |
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Target: 5'- gGCUCGCGGCACAGUUUCGAGAGGCCAc -3' miRNA: 3'- -CGAGCGCCGUGUCAAAGCUCUCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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