Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16505 | 3' | -60.4 | NC_004084.1 | + | 45234 | 0.66 | 0.471244 |
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Target: 5'- -uCCGUCaucacucgaUGUCCUcagagacgaAGCUGUACGUUCGg -3' miRNA: 3'- guGGCAG---------ACGGGG---------UCGACGUGCGAGC- -5' |
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| 16505 | 3' | -60.4 | NC_004084.1 | + | 44390 | 0.66 | 0.423789 |
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Target: 5'- aCACCGa-UGUCCacgAGCgcgGCugGCUCGg -3' miRNA: 3'- -GUGGCagACGGGg--UCGa--CGugCGAGC- -5' |
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| 16505 | 3' | -60.4 | NC_004084.1 | + | 47209 | 0.66 | 0.42287 |
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Target: 5'- cCACCGaucacccguccgcUCUGUCgCAGCUGCugGaaCGa -3' miRNA: 3'- -GUGGC-------------AGACGGgGUCGACGugCgaGC- -5' |
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| 16505 | 3' | -60.4 | NC_004084.1 | + | 5981 | 0.67 | 0.414645 |
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Target: 5'- aUACCGUgaGUagaCCCAGgUGaUACGCUCGc -3' miRNA: 3'- -GUGGCAgaCG---GGGUCgAC-GUGCGAGC- -5' |
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| 16505 | 3' | -60.4 | NC_004084.1 | + | 53972 | 0.67 | 0.387955 |
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Target: 5'- cCACUGUCugUGCUUCAGCUGCuucCGCg-- -3' miRNA: 3'- -GUGGCAG--ACGGGGUCGACGu--GCGagc -5' |
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| 16505 | 3' | -60.4 | NC_004084.1 | + | 42298 | 0.7 | 0.259175 |
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Target: 5'- uCACCGUCUucGUCUCGGCauacucaucUGCgagGCGCUCGg -3' miRNA: 3'- -GUGGCAGA--CGGGGUCG---------ACG---UGCGAGC- -5' |
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| 16505 | 3' | -60.4 | NC_004084.1 | + | 50810 | 0.72 | 0.195966 |
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Target: 5'- uCGCCGUC-GUagUCCAGCUGCugGCUg- -3' miRNA: 3'- -GUGGCAGaCG--GGGUCGACGugCGAgc -5' |
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| 16505 | 3' | -60.4 | NC_004084.1 | + | 58266 | 0.74 | 0.128199 |
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Target: 5'- cCACuaCGcCUGCCCUGGCUGCggGCGCUCu -3' miRNA: 3'- -GUG--GCaGACGGGGUCGACG--UGCGAGc -5' |
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| 16505 | 3' | -60.4 | NC_004084.1 | + | 58146 | 1.08 | 0.000383 |
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Target: 5'- uCACCGUCUGCCCCAGCUGCACGCUCGa -3' miRNA: 3'- -GUGGCAGACGGGGUCGACGUGCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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