miRNA: 3'- aGGCUuuUCGUGGCUacUUGCUgCUG- -5'
miRNA: 3'- aGGCUUUucGUGGCUA---------CUu--GCUGCUG- -5'
miRNA: 3'- aGGCU--------UUUCGUgGCUACUU-GCugCUG- -5'
miRNA: 3'- aGGCUu-UUC-GUGGCUACU-----UGCugCUG- -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16505 | 5' | -49.5 | NC_004084.1 | + | 30279 | 0.66 | 0.970936 |
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Target: 5'- gCCGA--AGUAUCGA--GACGAgGACg -3' miRNA: 3'- aGGCUuuUCGUGGCUacUUGCUgCUG- -5' |
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| 16505 | 5' | -49.5 | NC_004084.1 | + | 36530 | 0.66 | 0.970936 |
|
Target: 5'- aCCGAGA--CGCCGAUcgcgacaacGGuauCGACGACg -3' miRNA: 3'- aGGCUUUucGUGGCUA---------CUu--GCUGCUG- -5' |
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| 16505 | 5' | -49.5 | NC_004084.1 | + | 57631 | 0.66 | 0.970936 |
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Target: 5'- cUCGAccaacacaAGGGCGaCGAUGAAuCGugGACg -3' miRNA: 3'- aGGCU--------UUUCGUgGCUACUU-GCugCUG- -5' |
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| 16505 | 5' | -49.5 | NC_004084.1 | + | 45762 | 0.66 | 0.970936 |
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Target: 5'- cUCGAucGGGaCGCCGAUGGaggucAUGugGGCg -3' miRNA: 3'- aGGCUu-UUC-GUGGCUACU-----UGCugCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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