miRNA: 3'- aGGCU--------UUUCGUgGCUACUU-GCugCUG- -5'
miRNA: 3'- aGGCUUUUCGUGgcuacuuGCUGCUG- -5'
miRNA: 3'- aGGCuuUUCGUGGCuACuuGCUGCUG- -5'
miRNA: 3'- -AGGCUUUUCGUGGCUACUUGCUGCUG- -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16505 | 5' | -49.5 | NC_004084.1 | + | 57631 | 0.66 | 0.970936 |
|
Target: 5'- cUCGAccaacacaAGGGCGaCGAUGAAuCGugGACg -3' miRNA: 3'- aGGCU--------UUUCGUgGCUACUU-GCugCUG- -5' |
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| 16505 | 5' | -49.5 | NC_004084.1 | + | 57841 | 0.72 | 0.732384 |
|
Target: 5'- aCCGAAGAGCACau-----CGACGACa -3' miRNA: 3'- aGGCUUUUCGUGgcuacuuGCUGCUG- -5' |
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| 16505 | 5' | -49.5 | NC_004084.1 | + | 58059 | 0.76 | 0.530669 |
|
Target: 5'- cCCGguAGGCGCCG-UGuuCGACGGCg -3' miRNA: 3'- aGGCuuUUCGUGGCuACuuGCUGCUG- -5' |
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| 16505 | 5' | -49.5 | NC_004084.1 | + | 58180 | 1.11 | 0.003519 |
|
Target: 5'- cUCCGAAAAGCACCGAUGAACGACGACu -3' miRNA: 3'- -AGGCUUUUCGUGGCUACUUGCUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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