Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16506 | 5' | -56.7 | NC_004084.1 | + | 33115 | 0.66 | 0.631393 |
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Target: 5'- cGGCCUCUG-CGgCUaCUGCCGg--CGc -3' miRNA: 3'- aCCGGAGACaGUgGA-GACGGUagaGC- -5' |
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| 16506 | 5' | -56.7 | NC_004084.1 | + | 930 | 0.66 | 0.620455 |
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Target: 5'- cGGCCUgCUGgugaCGCUcccggaggaggUCgcggGCCAUCUCGc -3' miRNA: 3'- aCCGGA-GACa---GUGG-----------AGa---CGGUAGAGC- -5' |
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| 16506 | 5' | -56.7 | NC_004084.1 | + | 7099 | 0.67 | 0.576896 |
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Target: 5'- gGGCgcaCUCgaUCGCCUCggucGCCGUCUUGg -3' miRNA: 3'- aCCG---GAGacAGUGGAGa---CGGUAGAGC- -5' |
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| 16506 | 5' | -56.7 | NC_004084.1 | + | 7015 | 0.68 | 0.523505 |
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Target: 5'- aGGCCUCgacggcGUCGgC-C-GCCAUCUCGu -3' miRNA: 3'- aCCGGAGa-----CAGUgGaGaCGGUAGAGC- -5' |
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| 16506 | 5' | -56.7 | NC_004084.1 | + | 31942 | 0.7 | 0.423403 |
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Target: 5'- -uGCCUggGaCACCguugCUGCCGUCUCGa -3' miRNA: 3'- acCGGAgaCaGUGGa---GACGGUAGAGC- -5' |
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| 16506 | 5' | -56.7 | NC_004084.1 | + | 35745 | 0.76 | 0.167424 |
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Target: 5'- cUGGCCcCaG--GCCUCUGCCAUCUCGa -3' miRNA: 3'- -ACCGGaGaCagUGGAGACGGUAGAGC- -5' |
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| 16506 | 5' | -56.7 | NC_004084.1 | + | 58370 | 1.1 | 0.000644 |
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Target: 5'- uUGGCCUCUGUCACCUCUGCCAUCUCGg -3' miRNA: 3'- -ACCGGAGACAGUGGAGACGGUAGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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