miRNA: 3'- -AGCCAUAAACAGACGGUCACAUUAUC- -5'
miRNA: 3'- aGCCaua-AACAGACGGUCAcauuauc -5'
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16545 | 3' | -45.2 | NC_004105.1 | + | 19431 | 1.12 | 0.029271 |
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Target: 5'- aUCGGUAUUUGUCUGCCAGUGUAAUAGa -3' miRNA: 3'- -AGCCAUAAACAGACGGUCACAUUAUC- -5' |
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| 16545 | 3' | -45.2 | NC_004105.1 | + | 50344 | 0.66 | 0.999999 |
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Target: 5'- gCGGcuugUUGUCUGCCAGUu------ -3' miRNA: 3'- aGCCaua-AACAGACGGUCAcauuauc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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