Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16547 | 3' | -49.9 | NC_004105.1 | + | 176173 | 0.67 | 0.998054 |
Target: 5'- uUCGCGAcUGCuuCCGAAAuACAUGu- -3' miRNA: 3'- gAGCGCUcACGuuGGCUUU-UGUACcg -5' |
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16547 | 3' | -49.9 | NC_004105.1 | + | 184048 | 0.67 | 0.997255 |
Target: 5'- -aCGacaGGGUGUuuauUUGAGGGCAUGGCa -3' miRNA: 3'- gaGCg--CUCACGuu--GGCUUUUGUACCG- -5' |
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16547 | 3' | -49.9 | NC_004105.1 | + | 100856 | 0.68 | 0.9962 |
Target: 5'- uCUCcuaGAGUcgGCGACauuguaGAAAGCGUGGCc -3' miRNA: 3'- -GAGcg-CUCA--CGUUGg-----CUUUUGUACCG- -5' |
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16547 | 3' | -49.9 | NC_004105.1 | + | 173054 | 1.14 | 0.008396 |
Target: 5'- uCUCGCGAGUGCAACCGAAAACAUGGCg -3' miRNA: 3'- -GAGCGCUCACGUUGGCUUUUGUACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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