miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16558 3' -47.8 NC_004112.1 + 12065 0.66 0.960899
Target:  5'- uUCUGGUcGGUg--GAACaAGCUUgACCu -3'
miRNA:   3'- gAGACCAuUCGaaaCUUG-UCGAG-UGG- -5'
16558 3' -47.8 NC_004112.1 + 7932 0.7 0.816984
Target:  5'- gUC-GGUGucAGCUUUGAgaACAGCUCgauuaACCu -3'
miRNA:   3'- gAGaCCAU--UCGAAACU--UGUCGAG-----UGG- -5'
16558 3' -47.8 NC_004112.1 + 20140 0.71 0.785883
Target:  5'- ---cGGUAAuacGCUUgcUGGuACGGCUCACCa -3'
miRNA:   3'- gagaCCAUU---CGAA--ACU-UGUCGAGUGG- -5'
16558 3' -47.8 NC_004112.1 + 10169 0.73 0.684052
Target:  5'- -aCUGGUAua-UUUGcgUAGCUCACCg -3'
miRNA:   3'- gaGACCAUucgAAACuuGUCGAGUGG- -5'
16558 3' -47.8 NC_004112.1 + 32021 0.75 0.553987
Target:  5'- -gCUGGUGAGCUguucaaAGCUUACCa -3'
miRNA:   3'- gaGACCAUUCGAaacuugUCGAGUGG- -5'
16558 3' -47.8 NC_004112.1 + 32093 1.15 0.001627
Target:  5'- aCUCUGGUAAGCUUUGAACAGCUCACCa -3'
miRNA:   3'- -GAGACCAUUCGAAACUUGUCGAGUGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.