Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16559 | 3' | -46.8 | NC_004112.1 | + | 30046 | 0.67 | 0.965905 |
Target: 5'- aCCUUCGAcaacGCUUUACAGUAUCu--- -3' miRNA: 3'- -GGAGGUUau--CGAAAUGUCGUAGcaga -5' |
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16559 | 3' | -46.8 | NC_004112.1 | + | 11088 | 0.68 | 0.94856 |
Target: 5'- gCCUCaauuuGCUUgaUGCGuGCAUCGUCg -3' miRNA: 3'- -GGAGguuauCGAA--AUGU-CGUAGCAGa -5' |
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16559 | 3' | -46.8 | NC_004112.1 | + | 29197 | 0.7 | 0.882943 |
Target: 5'- aCUUUuuaAGCUUUGCGGCuUCGUCa -3' miRNA: 3'- gGAGGuuaUCGAAAUGUCGuAGCAGa -5' |
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16559 | 3' | -46.8 | NC_004112.1 | + | 12643 | 0.72 | 0.798474 |
Target: 5'- aCUCCAGcAGCaaucagcuUUUGCcuGGCAUCGUCa -3' miRNA: 3'- gGAGGUUaUCG--------AAAUG--UCGUAGCAGa -5' |
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16559 | 3' | -46.8 | NC_004112.1 | + | 33164 | 1.12 | 0.003703 |
Target: 5'- gCCUCCAAUAGCUUUACAGCAUCGUCUg -3' miRNA: 3'- -GGAGGUUAUCGAAAUGUCGUAGCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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