Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16559 | 5' | -44.6 | NC_004112.1 | + | 19551 | 0.66 | 0.998298 |
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Target: 5'- gGCAUuccgacuaguuuggACGCUGgcGGU-GCUGGAAcGCCg -3' miRNA: 3'- -CGUA--------------UGCGGCauUUAaUGGCCUU-UGG- -5' |
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| 16559 | 5' | -44.6 | NC_004112.1 | + | 28524 | 0.67 | 0.997093 |
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Target: 5'- gGCAUAauaGUCGUAAGgcgcuaggUACUGGuGAUCa -3' miRNA: 3'- -CGUAUg--CGGCAUUUa-------AUGGCCuUUGG- -5' |
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| 16559 | 5' | -44.6 | NC_004112.1 | + | 10672 | 0.67 | 0.994078 |
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Target: 5'- aGCAguCGUCGgugg-UGCCGGuguAGCCa -3' miRNA: 3'- -CGUauGCGGCauuuaAUGGCCu--UUGG- -5' |
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| 16559 | 5' | -44.6 | NC_004112.1 | + | 6152 | 0.67 | 0.994078 |
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Target: 5'- aGCuu-CGCUcaGAAUUGCCGGAAGa- -3' miRNA: 3'- -CGuauGCGGcaUUUAAUGGCCUUUgg -5' |
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| 16559 | 5' | -44.6 | NC_004112.1 | + | 4240 | 0.67 | 0.994078 |
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Target: 5'- cGgAUAUGCUGgcguGGUUACUGGAGAg- -3' miRNA: 3'- -CgUAUGCGGCau--UUAAUGGCCUUUgg -5' |
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| 16559 | 5' | -44.6 | NC_004112.1 | + | 37413 | 0.7 | 0.97236 |
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Target: 5'- ---cGCGCCu--GAUUagggacgcGCCGGAGGCCa -3' miRNA: 3'- cguaUGCGGcauUUAA--------UGGCCUUUGG- -5' |
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| 16559 | 5' | -44.6 | NC_004112.1 | + | 33092 | 0.72 | 0.937182 |
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Target: 5'- gGCAgacgAUGCUGUAAAgcUAUUGGAGGCUa -3' miRNA: 3'- -CGUa---UGCGGCAUUUa-AUGGCCUUUGG- -5' |
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| 16559 | 5' | -44.6 | NC_004112.1 | + | 26097 | 0.73 | 0.88168 |
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Target: 5'- cCAUACGCCaGU---UUGCUGGAGGCg -3' miRNA: 3'- cGUAUGCGG-CAuuuAAUGGCCUUUGg -5' |
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| 16559 | 5' | -44.6 | NC_004112.1 | + | 33130 | 1.18 | 0.003437 |
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Target: 5'- uGCAUACGCCGUAAAUUACCGGAAACCg -3' miRNA: 3'- -CGUAUGCGGCAUUUAAUGGCCUUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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