Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16564 | 3' | -52.8 | NC_004117.1 | + | 77053 | 0.66 | 0.981847 |
Target: 5'- cGCGgcaacgACGUGAgaguagcgaACCUAGCAUuUACGCa -3' miRNA: 3'- -CGCa-----UGCACUg--------UGGGUCGUGcAUGUG- -5' |
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16564 | 3' | -52.8 | NC_004117.1 | + | 38304 | 0.67 | 0.969227 |
Target: 5'- cCGUugGUGACGaugcguCCgCAGCACGUuCGu -3' miRNA: 3'- cGCAugCACUGU------GG-GUCGUGCAuGUg -5' |
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16564 | 3' | -52.8 | NC_004117.1 | + | 54911 | 0.67 | 0.969227 |
Target: 5'- aCGgacgACGaggaaGACACuCCAGCGCGU-CGCg -3' miRNA: 3'- cGCa---UGCa----CUGUG-GGUCGUGCAuGUG- -5' |
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16564 | 3' | -52.8 | NC_004117.1 | + | 49376 | 0.67 | 0.968924 |
Target: 5'- ---cGCG-GACccaacggACCCAGCAUGUACAg -3' miRNA: 3'- cgcaUGCaCUG-------UGGGUCGUGCAUGUg -5' |
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16564 | 3' | -52.8 | NC_004117.1 | + | 98470 | 0.67 | 0.962746 |
Target: 5'- gGUGUugaUGAgGCCUAGCAUGUGCGg -3' miRNA: 3'- -CGCAugcACUgUGGGUCGUGCAUGUg -5' |
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16564 | 3' | -52.8 | NC_004117.1 | + | 73919 | 0.68 | 0.951336 |
Target: 5'- cGUGUugacaaACuUGACAUCUucuucugugGGCACGUACACg -3' miRNA: 3'- -CGCA------UGcACUGUGGG---------UCGUGCAUGUG- -5' |
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16564 | 3' | -52.8 | NC_004117.1 | + | 141172 | 0.68 | 0.932796 |
Target: 5'- uUGUAUGUGACG-CCAGCGuCGUucCACu -3' miRNA: 3'- cGCAUGCACUGUgGGUCGU-GCAu-GUG- -5' |
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16564 | 3' | -52.8 | NC_004117.1 | + | 96420 | 0.69 | 0.90411 |
Target: 5'- aUGUGCGUcGACGCggAGC-CGUGCGCg -3' miRNA: 3'- cGCAUGCA-CUGUGggUCGuGCAUGUG- -5' |
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16564 | 3' | -52.8 | NC_004117.1 | + | 145236 | 0.69 | 0.90411 |
Target: 5'- cCGgauuCGUGAUACCCgcuGGCAUGUuCACg -3' miRNA: 3'- cGCau--GCACUGUGGG---UCGUGCAuGUG- -5' |
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16564 | 3' | -52.8 | NC_004117.1 | + | 9362 | 0.69 | 0.90411 |
Target: 5'- -aGUACGUGACAaa-AGCcACGUACAa -3' miRNA: 3'- cgCAUGCACUGUgggUCG-UGCAUGUg -5' |
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16564 | 3' | -52.8 | NC_004117.1 | + | 156424 | 0.71 | 0.845962 |
Target: 5'- uUGUGCGgucGugGCCguCGGCAUGUACGCc -3' miRNA: 3'- cGCAUGCa--CugUGG--GUCGUGCAUGUG- -5' |
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16564 | 3' | -52.8 | NC_004117.1 | + | 145041 | 0.74 | 0.675444 |
Target: 5'- gGUGUACGUGaACAUgCCAGCGgGUAuCACg -3' miRNA: 3'- -CGCAUGCAC-UGUG-GGUCGUgCAU-GUG- -5' |
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16564 | 3' | -52.8 | NC_004117.1 | + | 16456 | 1.12 | 0.004314 |
Target: 5'- gGCGUACGUGACACCCAGCACGUACACu -3' miRNA: 3'- -CGCAUGCACUGUGGGUCGUGCAUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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