Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16564 | 5' | -49.4 | NC_004117.1 | + | 54933 | 0.66 | 0.995889 |
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Target: 5'- ---cAGCGCGuCGCgugCGAAUGCGAACa- -3' miRNA: 3'- uucaUCGUGC-GCG---GCUUAUGUUUGac -5' |
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| 16564 | 5' | -49.4 | NC_004117.1 | + | 85705 | 0.67 | 0.994367 |
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Target: 5'- uGGUAuGCugGCGCCGAcauCAAugUu -3' miRNA: 3'- uUCAU-CGugCGCGGCUuauGUUugAc -5' |
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| 16564 | 5' | -49.4 | NC_004117.1 | + | 70611 | 0.67 | 0.992418 |
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Target: 5'- -cGUAGCACGCGUCGGuguCAAu--- -3' miRNA: 3'- uuCAUCGUGCGCGGCUuauGUUugac -5' |
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| 16564 | 5' | -49.4 | NC_004117.1 | + | 102022 | 0.67 | 0.992418 |
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Target: 5'- gGGGU-GCGCGgaCGCCGAGUACu-GCUa -3' miRNA: 3'- -UUCAuCGUGC--GCGGCUUAUGuuUGAc -5' |
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| 16564 | 5' | -49.4 | NC_004117.1 | + | 121357 | 0.67 | 0.991261 |
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Target: 5'- -uGUuGCACGCuuuaGCUGuGUGCGAGCUGu -3' miRNA: 3'- uuCAuCGUGCG----CGGCuUAUGUUUGAC- -5' |
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| 16564 | 5' | -49.4 | NC_004117.1 | + | 146712 | 0.68 | 0.989969 |
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Target: 5'- -cGUAGCACGCGUUuuGUACAGcACg- -3' miRNA: 3'- uuCAUCGUGCGCGGcuUAUGUU-UGac -5' |
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| 16564 | 5' | -49.4 | NC_004117.1 | + | 43787 | 0.69 | 0.97358 |
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Target: 5'- --aUAGCAUGCGUCGAuaccgcgGCGAACUc -3' miRNA: 3'- uucAUCGUGCGCGGCUua-----UGUUUGAc -5' |
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| 16564 | 5' | -49.4 | NC_004117.1 | + | 129217 | 0.7 | 0.967459 |
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Target: 5'- ---cGGUggGCGCCGcAGUGCAAAUUGg -3' miRNA: 3'- uucaUCGugCGCGGC-UUAUGUUUGAC- -5' |
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| 16564 | 5' | -49.4 | NC_004117.1 | + | 16422 | 1.07 | 0.013997 |
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Target: 5'- cAAGUAGCACGCGCCGAAUACAAACUGa -3' miRNA: 3'- -UUCAUCGUGCGCGGCUUAUGUUUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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