Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16566 | 5' | -49.7 | NC_004117.1 | + | 118411 | 0.71 | 0.91604 |
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Target: 5'- cGCGCGAucgauucugccaguCGCGUGCCGugGcAGCAu-- -3' miRNA: 3'- aCGCGUU--------------GUGCAUGGCugU-UCGUuuu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 131409 | 0.67 | 0.993731 |
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Target: 5'- uUGUGCAGCACacauCUGuCGAGCGGAu -3' miRNA: 3'- -ACGCGUUGUGcau-GGCuGUUCGUUUu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 133481 | 0.67 | 0.993731 |
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Target: 5'- uUGCGC-GCACGUGCCGuCcucGGCc--- -3' miRNA: 3'- -ACGCGuUGUGCAUGGCuGu--UCGuuuu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 133513 | 0.67 | 0.991618 |
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Target: 5'- cGCGacaGAUACGauaugGgCGACAGGCAGAu -3' miRNA: 3'- aCGCg--UUGUGCa----UgGCUGUUCGUUUu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 135661 | 0.67 | 0.988978 |
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Target: 5'- gGCGUAcgccccugGCAucuauUGUGCCGACuuuAGCAAGAu -3' miRNA: 3'- aCGCGU--------UGU-----GCAUGGCUGu--UCGUUUU- -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 141225 | 0.68 | 0.981799 |
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Target: 5'- cGCGCGACACGUuUCG-CA-GCAGu- -3' miRNA: 3'- aCGCGUUGUGCAuGGCuGUuCGUUuu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 142093 | 0.73 | 0.861126 |
|
Target: 5'- -aCGCuucuGACA-GUGCCGACGAGCAAAAc -3' miRNA: 3'- acGCG----UUGUgCAUGGCUGUUCGUUUU- -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 151932 | 0.71 | 0.923787 |
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Target: 5'- aGUGUGGCACGUAUuCGGCGuguGCAAGAg -3' miRNA: 3'- aCGCGUUGUGCAUG-GCUGUu--CGUUUU- -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 156051 | 0.66 | 0.996678 |
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Target: 5'- cGCGCuuccaaccguACAUGUgcACCGACGccauuuuaGGCAAAGu -3' miRNA: 3'- aCGCGu---------UGUGCA--UGGCUGU--------UCGUUUU- -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 157555 | 0.66 | 0.994531 |
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Target: 5'- gGUGCGuCACGUggaucaaGgUGGCAAGCGAAAa -3' miRNA: 3'- aCGCGUuGUGCA-------UgGCUGUUCGUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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