miRNA: 3'- -ACUGCUCacgCUAuUCGCGC-CCGCUG- -5'
miRNA: 3'- acUGC-UCACGcUAUUcGCGCCCGCUG- -5'
miRNA: 3'- -ACUGCUCACGC-UAUu------CGCGCccgCUG- -5'
miRNA: 3'- aCUGCUCACGCUaUUCGCgcCCGCuG- -5'
miRNA: 3'- -ACUGCUCACGCUAUUCGCGCCCGCUG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16567 | 5' | -56 | NC_004117.1 | + | 116884 | 0.66 | 0.91993 |
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Target: 5'- gUGACGAGga-GAU-GGUGUGuGGCGAUc -3' miRNA: 3'- -ACUGCUCacgCUAuUCGCGC-CCGCUG- -5' |
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| 16567 | 5' | -56 | NC_004117.1 | + | 138560 | 0.66 | 0.908218 |
|
Target: 5'- -aAUGcAGUGCuAUGAaCGUGGGCGGCa -3' miRNA: 3'- acUGC-UCACGcUAUUcGCGCCCGCUG- -5' |
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| 16567 | 5' | -56 | NC_004117.1 | + | 141614 | 0.67 | 0.874881 |
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Target: 5'- cUGACGAGUGCGuAUAcuucgcuGCGCGucaGACa -3' miRNA: 3'- -ACUGCUCACGC-UAUu------CGCGCccgCUG- -5' |
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| 16567 | 5' | -56 | NC_004117.1 | + | 6105 | 0.77 | 0.339443 |
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Target: 5'- aGACGGGUGCGGcGGGCGauGGCGuCg -3' miRNA: 3'- aCUGCUCACGCUaUUCGCgcCCGCuG- -5' |
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| 16567 | 5' | -56 | NC_004117.1 | + | 19443 | 1.1 | 0.002423 |
|
Target: 5'- uUGACGAGUGCGAUAAGCGCGGGCGACg -3' miRNA: 3'- -ACUGCUCACGCUAUUCGCGCCCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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