Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16569 | 3' | -51.9 | NC_004117.1 | + | 110741 | 0.66 | 0.989799 |
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Target: 5'- ---aGCAUUgucgCGAGCUGCUCG-CUAa -3' miRNA: 3'- cuagCGUAGa---GCUUGGCGAGCaGAUg -5' |
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| 16569 | 3' | -51.9 | NC_004117.1 | + | 30133 | 0.66 | 0.988394 |
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Target: 5'- cAUCGCGUaUCGGgauuGCCGCaaGUUUGCa -3' miRNA: 3'- cUAGCGUAgAGCU----UGGCGagCAGAUG- -5' |
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| 16569 | 3' | -51.9 | NC_004117.1 | + | 1153 | 0.66 | 0.98327 |
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Target: 5'- uGUUGCAcuUCUUGAACCGCUUuuuugUUACg -3' miRNA: 3'- cUAGCGU--AGAGCUUGGCGAGca---GAUG- -5' |
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| 16569 | 3' | -51.9 | NC_004117.1 | + | 42198 | 0.68 | 0.957739 |
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Target: 5'- cGUCGCGUUccaUCGGGCaacaGCUCGUCc-- -3' miRNA: 3'- cUAGCGUAG---AGCUUGg---CGAGCAGaug -5' |
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| 16569 | 3' | -51.9 | NC_004117.1 | + | 125940 | 0.69 | 0.925048 |
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Target: 5'- cGAUCGCAUCau--ACCGgUUCGUUUACg -3' miRNA: 3'- -CUAGCGUAGagcuUGGC-GAGCAGAUG- -5' |
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| 16569 | 3' | -51.9 | NC_004117.1 | + | 94953 | 0.7 | 0.907292 |
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Target: 5'- aAUUGCAUCUUuaAugUGUUCGUCUGCa -3' miRNA: 3'- cUAGCGUAGAGc-UugGCGAGCAGAUG- -5' |
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| 16569 | 3' | -51.9 | NC_004117.1 | + | 20117 | 1.1 | 0.005915 |
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Target: 5'- gGAUCGCAUCUCGAACCGCUCGUCUACg -3' miRNA: 3'- -CUAGCGUAGAGCUUGGCGAGCAGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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