Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16569 | 5' | -43 | NC_004117.1 | + | 99020 | 0.66 | 1 |
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Target: 5'- cAACAAUuGACGGaguuggcgUUCGAgaacGAAGCGUUg -3' miRNA: 3'- uUUGUUAuUUGCC--------AAGCU----UUUCGCGA- -5' |
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| 16569 | 5' | -43 | NC_004117.1 | + | 108126 | 0.66 | 1 |
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Target: 5'- uGAACAGUAuuugcgcucacguCGGUauucuuguaUCGAGGAGUGCg -3' miRNA: 3'- -UUUGUUAUuu-----------GCCA---------AGCUUUUCGCGa -5' |
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| 16569 | 5' | -43 | NC_004117.1 | + | 126950 | 0.67 | 0.999999 |
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Target: 5'- uGGCGAUAGACGGUgCGAugacacAGCGa- -3' miRNA: 3'- uUUGUUAUUUGCCAaGCUuu----UCGCga -5' |
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| 16569 | 5' | -43 | NC_004117.1 | + | 124342 | 0.69 | 0.999989 |
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Target: 5'- aAAGCGucuAUAAuuguuuugaucACGGUUCGAAuGGUGCUc -3' miRNA: 3'- -UUUGU---UAUU-----------UGCCAAGCUUuUCGCGA- -5' |
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| 16569 | 5' | -43 | NC_004117.1 | + | 77988 | 0.7 | 0.999942 |
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Target: 5'- aGAGCAuacAACGGUUUGuaccuGGGCGCUu -3' miRNA: 3'- -UUUGUuauUUGCCAAGCuu---UUCGCGA- -5' |
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| 16569 | 5' | -43 | NC_004117.1 | + | 19925 | 0.7 | 0.999893 |
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Target: 5'- gAGAUAAUcguagacGAGCGGUUCGAGAuGCGa- -3' miRNA: 3'- -UUUGUUA-------UUUGCCAAGCUUUuCGCga -5' |
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| 16569 | 5' | -43 | NC_004117.1 | + | 1340 | 0.71 | 0.99982 |
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Target: 5'- uAACAAaaAAGCGGUUCaAGAAGUGCa -3' miRNA: 3'- uUUGUUa-UUUGCCAAGcUUUUCGCGa -5' |
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| 16569 | 5' | -43 | NC_004117.1 | + | 108986 | 0.74 | 0.997403 |
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Target: 5'- gAAAUAAUGugaucGAUGGcugcaUUCGAAAAGCGCUg -3' miRNA: 3'- -UUUGUUAU-----UUGCC-----AAGCUUUUCGCGA- -5' |
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| 16569 | 5' | -43 | NC_004117.1 | + | 20080 | 1.06 | 0.075844 |
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Target: 5'- aAAACAAUAAACGGUUCGAAAAGCGCUc -3' miRNA: 3'- -UUUGUUAUUUGCCAAGCUUUUCGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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