Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16572 | 3' | -51.4 | NC_004117.1 | + | 122099 | 0.66 | 0.990516 |
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Target: 5'- -aAGUG-UCAUCGUCGUcGUCguCGUCGg -3' miRNA: 3'- uaUUACuAGUGGCAGCGaCAG--GCAGC- -5' |
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| 16572 | 3' | -51.4 | NC_004117.1 | + | 78648 | 0.66 | 0.989185 |
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Target: 5'- -cAGUGGaUACCGUCGUcGUCCGg-- -3' miRNA: 3'- uaUUACUaGUGGCAGCGaCAGGCagc -5' |
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| 16572 | 3' | -51.4 | NC_004117.1 | + | 97841 | 0.66 | 0.987712 |
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Target: 5'- uUAAUGA-CAUUGguguuuUCGCUGUUCGUCa -3' miRNA: 3'- uAUUACUaGUGGC------AGCGACAGGCAGc -5' |
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| 16572 | 3' | -51.4 | NC_004117.1 | + | 77793 | 0.67 | 0.986089 |
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Target: 5'- aAUAAUGGUCGCgG-CGCgGUCguUGUCGa -3' miRNA: 3'- -UAUUACUAGUGgCaGCGaCAG--GCAGC- -5' |
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| 16572 | 3' | -51.4 | NC_004117.1 | + | 135972 | 0.67 | 0.984307 |
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Target: 5'- -------cCAUCGUCGCUGaugcgCCGUCGu -3' miRNA: 3'- uauuacuaGUGGCAGCGACa----GGCAGC- -5' |
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| 16572 | 3' | -51.4 | NC_004117.1 | + | 45154 | 0.67 | 0.982356 |
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Target: 5'- gGUAAUGAUgGUCGUCGUaaUGuUCCGUCc -3' miRNA: 3'- -UAUUACUAgUGGCAGCG--AC-AGGCAGc -5' |
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| 16572 | 3' | -51.4 | NC_004117.1 | + | 72576 | 0.67 | 0.977916 |
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Target: 5'- -cGGUGG----UGUCGUUGUCCGUCGa -3' miRNA: 3'- uaUUACUagugGCAGCGACAGGCAGC- -5' |
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| 16572 | 3' | -51.4 | NC_004117.1 | + | 29234 | 0.68 | 0.9727 |
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Target: 5'- -aGcgGGUCGuuGUCGUUGccccgggCCGUCGa -3' miRNA: 3'- uaUuaCUAGUggCAGCGACa------GGCAGC- -5' |
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| 16572 | 3' | -51.4 | NC_004117.1 | + | 97708 | 0.68 | 0.969783 |
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Target: 5'- uUGGUG-UCugUGUCGUUGU-CGUCGg -3' miRNA: 3'- uAUUACuAGugGCAGCGACAgGCAGC- -5' |
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| 16572 | 3' | -51.4 | NC_004117.1 | + | 58168 | 0.71 | 0.883726 |
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Target: 5'- ----aGAUCACCGUCGCcGagaCGUCGu -3' miRNA: 3'- uauuaCUAGUGGCAGCGaCag-GCAGC- -5' |
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| 16572 | 3' | -51.4 | NC_004117.1 | + | 59267 | 1.07 | 0.011402 |
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Target: 5'- aAUAAUGAUCACCGUCGCUGUCCGUCGu -3' miRNA: 3'- -UAUUACUAGUGGCAGCGACAGGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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