Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16572 | 5' | -48.9 | NC_004117.1 | + | 132184 | 0.66 | 0.99872 |
Target: 5'- uGCCGAuGCGAuauuUUAGgGAgGGCGAAUu -3' miRNA: 3'- -CGGCUuCGCU----AGUUgCUgUCGUUUGu -5' |
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16572 | 5' | -48.9 | NC_004117.1 | + | 132244 | 0.75 | 0.842476 |
Target: 5'- cGgCGGAGaCGAUCAA-GACGGCGAACu -3' miRNA: 3'- -CgGCUUC-GCUAGUUgCUGUCGUUUGu -5' |
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16572 | 5' | -48.9 | NC_004117.1 | + | 133005 | 0.7 | 0.979224 |
Target: 5'- gGCCGgcGUGGuaguguccaacgUCAaaauuggcuggauuuACGACGGCAAACGa -3' miRNA: 3'- -CGGCuuCGCU------------AGU---------------UGCUGUCGUUUGU- -5' |
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16572 | 5' | -48.9 | NC_004117.1 | + | 135931 | 0.66 | 0.998643 |
Target: 5'- aGCCGAAguugcuuacgagcuGCGAgaaAGCGGCA-CAGGCAu -3' miRNA: 3'- -CGGCUU--------------CGCUag-UUGCUGUcGUUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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