Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16574 | 3' | -48 | NC_004117.1 | + | 45313 | 0.66 | 0.999113 |
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Target: 5'- cGUUGGAaucgccaacUugACUGCCGUUcACGGUCu -3' miRNA: 3'- -UAACCU---------AugUGAUGGCGGaUGUUAGu -5' |
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| 16574 | 3' | -48 | NC_004117.1 | + | 45876 | 0.68 | 0.99667 |
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Target: 5'- -gUGGAgcACACgACCGCCUugGAa-- -3' miRNA: 3'- uaACCUa-UGUGaUGGCGGAugUUagu -5' |
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| 16574 | 3' | -48 | NC_004117.1 | + | 41554 | 0.68 | 0.99667 |
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Target: 5'- ----aGUGCACUACCGCacaUACAAUUc -3' miRNA: 3'- uaaccUAUGUGAUGGCGg--AUGUUAGu -5' |
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| 16574 | 3' | -48 | NC_004117.1 | + | 19387 | 0.68 | 0.99667 |
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Target: 5'- cGUUGGGguucGCGCcauUGCCGCCUGgaaacuccCAAUCGa -3' miRNA: 3'- -UAACCUa---UGUG---AUGGCGGAU--------GUUAGU- -5' |
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| 16574 | 3' | -48 | NC_004117.1 | + | 70013 | 0.68 | 0.994575 |
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Target: 5'- --cGGAU-UACcGCCGCCUuugcGCAAUCGg -3' miRNA: 3'- uaaCCUAuGUGaUGGCGGA----UGUUAGU- -5' |
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| 16574 | 3' | -48 | NC_004117.1 | + | 75346 | 1.08 | 0.018498 |
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Target: 5'- uAUUGGAUACACUACCGCCUACAAUCAg -3' miRNA: 3'- -UAACCUAUGUGAUGGCGGAUGUUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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