miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16574 5' -44 NC_004117.1 + 10396 0.66 1
Target:  5'- --cGUUGAcGCUCGA--GAGUAugCAc -3'
miRNA:   3'- ucaCAGCUaUGAGCUauUUCAUugGU- -5'
16574 5' -44 NC_004117.1 + 79836 0.67 0.999997
Target:  5'- uAGUGUCGAU-CUCGAUAAcacacUGAUUAg -3'
miRNA:   3'- -UCACAGCUAuGAGCUAUUuc---AUUGGU- -5'
16574 5' -44 NC_004117.1 + 10592 0.67 0.999996
Target:  5'- cGGUGUCGAUGaggccgugcauacuCUCGAgcgucaacggugAAGGUAcagGCCAg -3'
miRNA:   3'- -UCACAGCUAU--------------GAGCUa-----------UUUCAU---UGGU- -5'
16574 5' -44 NC_004117.1 + 130146 0.69 0.999975
Target:  5'- -aUGUUGGUGCUUGA--GAGUuuGACCGa -3'
miRNA:   3'- ucACAGCUAUGAGCUauUUCA--UUGGU- -5'
16574 5' -44 NC_004117.1 + 23410 0.72 0.998564
Target:  5'- --aGUCGAUGCgguugCGGgucAAGUGACCAc -3'
miRNA:   3'- ucaCAGCUAUGa----GCUau-UUCAUUGGU- -5'
16574 5' -44 NC_004117.1 + 75310 1.11 0.03788
Target:  5'- uAGUGUCGAUACUCGAUAAAGUAACCAa -3'
miRNA:   3'- -UCACAGCUAUGAGCUAUUUCAUUGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.