Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16574 | 5' | -44 | NC_004117.1 | + | 10396 | 0.66 | 1 |
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Target: 5'- --cGUUGAcGCUCGA--GAGUAugCAc -3' miRNA: 3'- ucaCAGCUaUGAGCUauUUCAUugGU- -5' |
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| 16574 | 5' | -44 | NC_004117.1 | + | 79836 | 0.67 | 0.999997 |
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Target: 5'- uAGUGUCGAU-CUCGAUAAcacacUGAUUAg -3' miRNA: 3'- -UCACAGCUAuGAGCUAUUuc---AUUGGU- -5' |
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| 16574 | 5' | -44 | NC_004117.1 | + | 10592 | 0.67 | 0.999996 |
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Target: 5'- cGGUGUCGAUGaggccgugcauacuCUCGAgcgucaacggugAAGGUAcagGCCAg -3' miRNA: 3'- -UCACAGCUAU--------------GAGCUa-----------UUUCAU---UGGU- -5' |
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| 16574 | 5' | -44 | NC_004117.1 | + | 130146 | 0.69 | 0.999975 |
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Target: 5'- -aUGUUGGUGCUUGA--GAGUuuGACCGa -3' miRNA: 3'- ucACAGCUAUGAGCUauUUCA--UUGGU- -5' |
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| 16574 | 5' | -44 | NC_004117.1 | + | 23410 | 0.72 | 0.998564 |
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Target: 5'- --aGUCGAUGCgguugCGGgucAAGUGACCAc -3' miRNA: 3'- ucaCAGCUAUGa----GCUau-UUCAUUGGU- -5' |
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| 16574 | 5' | -44 | NC_004117.1 | + | 75310 | 1.11 | 0.03788 |
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Target: 5'- uAGUGUCGAUACUCGAUAAAGUAACCAa -3' miRNA: 3'- -UCACAGCUAUGAGCUAUUUCAUUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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