Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16575 | 3' | -54 | NC_004117.1 | + | 69756 | 0.66 | 0.9675 |
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Target: 5'- -aUCCGGCcaaauuguugaacgACACg-ACGUCACCGAa -3' miRNA: 3'- ccAGGCCGa-------------UGUGgaUGUAGUGGCUg -5' |
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| 16575 | 3' | -54 | NC_004117.1 | + | 60685 | 0.66 | 0.962929 |
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Target: 5'- cGGUCC-GCUcauuUACUUcACAUCACCGGg -3' miRNA: 3'- -CCAGGcCGAu---GUGGA-UGUAGUGGCUg -5' |
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| 16575 | 3' | -54 | NC_004117.1 | + | 19139 | 0.66 | 0.955651 |
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Target: 5'- cGGgcaCGGC--CACCgguccCGUCGCCGGCa -3' miRNA: 3'- -CCag-GCCGauGUGGau---GUAGUGGCUG- -5' |
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| 16575 | 3' | -54 | NC_004117.1 | + | 131237 | 0.66 | 0.955651 |
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Target: 5'- cGUCCGucaucguGCCUACGUCGCuCGACa -3' miRNA: 3'- cCAGGCcgaug--UGGAUGUAGUG-GCUG- -5' |
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| 16575 | 3' | -54 | NC_004117.1 | + | 78729 | 0.67 | 0.943011 |
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Target: 5'- cGUCCGcuGCUGCugCUGCGaCGCCu-- -3' miRNA: 3'- cCAGGC--CGAUGugGAUGUaGUGGcug -5' |
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| 16575 | 3' | -54 | NC_004117.1 | + | 148855 | 0.69 | 0.885352 |
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Target: 5'- -cUCgCGGCUACAUUaaaagcgggUACAUCAUCGAUg -3' miRNA: 3'- ccAG-GCCGAUGUGG---------AUGUAGUGGCUG- -5' |
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| 16575 | 3' | -54 | NC_004117.1 | + | 70171 | 0.71 | 0.768716 |
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Target: 5'- uGGUcgaaCCGGUUGCGUC-GCGUCGCCGGCc -3' miRNA: 3'- -CCA----GGCCGAUGUGGaUGUAGUGGCUG- -5' |
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| 16575 | 3' | -54 | NC_004117.1 | + | 96388 | 1.06 | 0.007804 |
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Target: 5'- cGGUgCCGGCUACACCUACAUCACCGACg -3' miRNA: 3'- -CCA-GGCCGAUGUGGAUGUAGUGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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