Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16578 | 3' | -62.1 | NC_004117.1 | + | 144653 | 0.66 | 0.641381 |
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Target: 5'- gGCgCGUUGUuuGgCCGCaACCuCGUUUCCAa -3' miRNA: 3'- -CG-GUAGCGggC-GGCG-UGG-GCAGAGGU- -5' |
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| 16578 | 3' | -62.1 | NC_004117.1 | + | 80203 | 0.67 | 0.621864 |
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Target: 5'- aGUCGUUGUCCGCCGCAUaugguaaCGUCa--- -3' miRNA: 3'- -CGGUAGCGGGCGGCGUGg------GCAGaggu -5' |
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| 16578 | 3' | -62.1 | NC_004117.1 | + | 6011 | 0.68 | 0.563687 |
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Target: 5'- aGCUGUUGuaCCUGCCGUGCCgGaCUCCAc -3' miRNA: 3'- -CGGUAGC--GGGCGGCGUGGgCaGAGGU- -5' |
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| 16578 | 3' | -62.1 | NC_004117.1 | + | 19256 | 0.71 | 0.36274 |
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Target: 5'- aUCGUCGCCCGCgcuuauCGCACUCGUCaaCAg -3' miRNA: 3'- cGGUAGCGGGCG------GCGUGGGCAGagGU- -5' |
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| 16578 | 3' | -62.1 | NC_004117.1 | + | 78792 | 0.73 | 0.27944 |
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Target: 5'- uUCGUCGCCUGCgCGCAUuuGuuUCUCCAu -3' miRNA: 3'- cGGUAGCGGGCG-GCGUGggC--AGAGGU- -5' |
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| 16578 | 3' | -62.1 | NC_004117.1 | + | 5925 | 1.1 | 0.000794 |
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Target: 5'- cGCCAUCGCCCGCCGCACCCGUCUCCAg -3' miRNA: 3'- -CGGUAGCGGGCGGCGUGGGCAGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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