miRNA: 3'- uauGCAGCua-UAUAGUCUCa---------ACGAUG- -5'
miRNA: 3'- uaUGCAGCUauaUAGUCUCAACGA-UG- -5'
miRNA: 3'- -UAUGC----AGCUa---UAUAguCUCaACGAUG- -5'
miRNA: 3'- uAUGCAGCUAUAua---GUCUCAAC-GAUG- -5'
miRNA: 3'- -UAUGCAGCUAUAUAGUCUCAACGAUG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16579 | 5' | -44.8 | NC_004117.1 | + | 97693 | 0.66 | 0.999995 |
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Target: 5'- --uUGUCGucgGUGUCGGAGgaagaugaacUGCUGCu -3' miRNA: 3'- uauGCAGCua-UAUAGUCUCa---------ACGAUG- -5' |
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| 16579 | 5' | -44.8 | NC_004117.1 | + | 7535 | 0.69 | 0.999729 |
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Target: 5'- -gACGUCGGc--GUUAGGGUUGCUcGCc -3' miRNA: 3'- uaUGCAGCUauaUAGUCUCAACGA-UG- -5' |
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| 16579 | 5' | -44.8 | NC_004117.1 | + | 64703 | 0.7 | 0.999305 |
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Target: 5'- cGUACGcgaaUCGAgucgGUGUguGAGcUGCUGCg -3' miRNA: 3'- -UAUGC----AGCUa---UAUAguCUCaACGAUG- -5' |
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| 16579 | 5' | -44.8 | NC_004117.1 | + | 120189 | 0.76 | 0.959064 |
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Target: 5'- uUGCGUCGAUAUcucgaCAGAGUUGuUUGCa -3' miRNA: 3'- uAUGCAGCUAUAua---GUCUCAAC-GAUG- -5' |
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| 16579 | 5' | -44.8 | NC_004117.1 | + | 7388 | 1.08 | 0.037894 |
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Target: 5'- aAUACGUCGAUAUAUCAGAGUUGCUACc -3' miRNA: 3'- -UAUGCAGCUAUAUAGUCUCAACGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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