Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 1658 | 3' | -55.4 | NC_001347.2 | + | 140575 | 0.73 | 0.72784 |
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Target: 5'- -cGGGcggcggugcuGGCGggGGugGUGGACGAgACg -3' miRNA: 3'- gaCCC----------CUGCuuCUugCGCCUGCUgUG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 112783 | 0.74 | 0.629798 |
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Target: 5'- -cGGGGGCGccguAGGACGCGGGauaaGACGg -3' miRNA: 3'- gaCCCCUGCu---UCUUGCGCCUg---CUGUg -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 40729 | 0.75 | 0.60007 |
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Target: 5'- uUGGGGACGggGAcaguuugcgGCGCGGACc---- -3' miRNA: 3'- gACCCCUGCuuCU---------UGCGCCUGcugug -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 177647 | 0.75 | 0.58035 |
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Target: 5'- -cGGGGA-GGAGAGCGUaacGGAgGACACa -3' miRNA: 3'- gaCCCCUgCUUCUUGCG---CCUgCUGUG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 182477 | 0.75 | 0.590196 |
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Target: 5'- gCUGaGGacgacGACGAAG-ACGCGGACGACGa -3' miRNA: 3'- -GAC-CC-----CUGCUUCuUGCGCCUGCUGUg -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 113162 | 0.76 | 0.540413 |
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Target: 5'- -cGGGGACGAAGAgggaACGCGcgccccagccgccGGCGugGCg -3' miRNA: 3'- gaCCCCUGCUUCU----UGCGC-------------CUGCugUG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 1972 | 0.76 | 0.560771 |
|
Target: 5'- -cGGGGACGgcG-GCGgGGACGACGu -3' miRNA: 3'- gaCCCCUGCuuCuUGCgCCUGCUGUg -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 39513 | 0.81 | 0.299914 |
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Target: 5'- --aGGGGCGggGGGCGCGG-CGACAUg -3' miRNA: 3'- gacCCCUGCuuCUUGCGCCuGCUGUG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 117980 | 1.1 | 0.004625 |
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Target: 5'- cCUGGGGACGAAGAACGCGGACGACACg -3' miRNA: 3'- -GACCCCUGCUUCUUGCGCCUGCUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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