Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1658 | 3' | -55.4 | NC_001347.2 | + | 157968 | 0.69 | 0.885443 |
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Target: 5'- aCUGGGucugcacGACGAGacGCGCGGAgGAgGCu -3' miRNA: 3'- -GACCC-------CUGCUUcuUGCGCCUgCUgUG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 164904 | 0.69 | 0.892679 |
|
Target: 5'- -gGGGGAgGAGGAG-GCGGugGcCAg -3' miRNA: 3'- gaCCCCUgCUUCUUgCGCCugCuGUg -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 173654 | 0.66 | 0.973401 |
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Target: 5'- uCUGGGa--GGAGGugGCGGcggugGCGGCAa -3' miRNA: 3'- -GACCCcugCUUCUugCGCC-----UGCUGUg -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 177647 | 0.75 | 0.58035 |
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Target: 5'- -cGGGGA-GGAGAGCGUaacGGAgGACACa -3' miRNA: 3'- gaCCCCUgCUUCUUGCG---CCUgCUGUG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 182477 | 0.75 | 0.590196 |
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Target: 5'- gCUGaGGacgacGACGAAG-ACGCGGACGACGa -3' miRNA: 3'- -GAC-CC-----CUGCUUCuUGCGCCUGCUGUg -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 184908 | 0.66 | 0.975912 |
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Target: 5'- gCUGGu--UGAAGAGCGCGcacacgcGCGACACa -3' miRNA: 3'- -GACCccuGCUUCUUGCGCc------UGCUGUG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 192047 | 0.71 | 0.801064 |
|
Target: 5'- -cGGcGACGAcGGugGCGGcgACGACACg -3' miRNA: 3'- gaCCcCUGCUuCUugCGCC--UGCUGUG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 201509 | 0.66 | 0.975912 |
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Target: 5'- uUGGugcgcGACGAAGGACG-GGAUG-CACa -3' miRNA: 3'- gACCc----CUGCUUCUUGCgCCUGCuGUG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 228023 | 0.69 | 0.905167 |
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Target: 5'- gUGGGGACGGAuucuuGAggcuacaaagauACGCGGAgacguCGugGCg -3' miRNA: 3'- gACCCCUGCUU-----CU------------UGCGCCU-----GCugUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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