Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1658 | 3' | -55.4 | NC_001347.2 | + | 107436 | 0.67 | 0.950355 |
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Target: 5'- -cGGcGGACGAGGGAC-UGGAgGuGCGCg -3' miRNA: 3'- gaCC-CCUGCUUCUUGcGCCUgC-UGUG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 12139 | 0.67 | 0.954268 |
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Target: 5'- uCUGaGGACGAGG-ACuCGGACGAUgACg -3' miRNA: 3'- -GACcCCUGCUUCuUGcGCCUGCUG-UG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 17221 | 0.67 | 0.953117 |
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Target: 5'- uUGGcGGACGAugaggagcagggagAGGAUGauGACGACAg -3' miRNA: 3'- gACC-CCUGCU--------------UCUUGCgcCUGCUGUg -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 52058 | 0.67 | 0.957967 |
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Target: 5'- aCU-GGGACGAGGAGgaggaggcgucCGCGGcccGCGAgCGCa -3' miRNA: 3'- -GAcCCCUGCUUCUU-----------GCGCC---UGCU-GUG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 33762 | 0.67 | 0.957967 |
|
Target: 5'- -cGGGGcCGuuuGGAACGCGuuucgcuugauaGAaCGACACg -3' miRNA: 3'- gaCCCCuGCu--UCUUGCGC------------CU-GCUGUG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 109361 | 0.66 | 0.964739 |
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Target: 5'- cCUGGGaccggcacGACGAGGGugcCGCccaGGGCGACGa -3' miRNA: 3'- -GACCC--------CUGCUUCUu--GCG---CCUGCUGUg -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 63062 | 0.66 | 0.964739 |
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Target: 5'- aUGGGuGGCGGAGGACugccgGCGGGugugggcuCGGCAa -3' miRNA: 3'- gACCC-CUGCUUCUUG-----CGCCU--------GCUGUg -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 130635 | 0.66 | 0.964739 |
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Target: 5'- gCUGGaagaGGACGAAGAcaGUG-ACGACGCu -3' miRNA: 3'- -GACC----CCUGCUUCUugCGCcUGCUGUG- -5' |
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| 1658 | 3' | -55.4 | NC_001347.2 | + | 151256 | 0.66 | 0.975912 |
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Target: 5'- -aGGGGGCucGAAcGAACgGCGGGuggaGGCGCg -3' miRNA: 3'- gaCCCCUG--CUU-CUUG-CGCCUg---CUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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