Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16580 | 3' | -51.1 | NC_004117.1 | + | 27791 | 0.66 | 0.992168 |
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Target: 5'- -aGAGGugUCAUua--CGUACGGCUCg -3' miRNA: 3'- ugUUUCugAGUGugagGUAUGCCGGG- -5' |
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| 16580 | 3' | -51.1 | NC_004117.1 | + | 34349 | 0.67 | 0.988167 |
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Target: 5'- uCAAAGACUCGCAgcagcauUUCUAUAaacucUGGCuCCa -3' miRNA: 3'- uGUUUCUGAGUGU-------GAGGUAU-----GCCG-GG- -5' |
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| 16580 | 3' | -51.1 | NC_004117.1 | + | 99165 | 0.68 | 0.970904 |
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Target: 5'- gACAuuGACggucaagUACAUUCCAUuaaaauUGGCCCu -3' miRNA: 3'- -UGUuuCUGa------GUGUGAGGUAu-----GCCGGG- -5' |
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| 16580 | 3' | -51.1 | NC_004117.1 | + | 129262 | 0.69 | 0.961049 |
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Target: 5'- gAUGGAGGCUauaACACUUCAaACGGCaCUg -3' miRNA: 3'- -UGUUUCUGAg--UGUGAGGUaUGCCG-GG- -5' |
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| 16580 | 3' | -51.1 | NC_004117.1 | + | 78697 | 0.71 | 0.912493 |
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Target: 5'- cGCAGGGGCUCuucgaaUCCGUACGGUUa -3' miRNA: 3'- -UGUUUCUGAGugug--AGGUAUGCCGGg -5' |
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| 16580 | 3' | -51.1 | NC_004117.1 | + | 18231 | 1.13 | 0.004673 |
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Target: 5'- gACAAAGACUCACACUCCAUACGGCCCa -3' miRNA: 3'- -UGUUUCUGAGUGUGAGGUAUGCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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