Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16581 | 5' | -55 | NC_004117.1 | + | 149052 | 0.66 | 0.93111 |
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Target: 5'- -uGGGCAUCGaUGAUGUacccgcuuuuaaUGUaGCCGCg -3' miRNA: 3'- cuCCCGUAGUaACUACG------------GCAgCGGUG- -5' |
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| 16581 | 5' | -55 | NC_004117.1 | + | 34593 | 0.66 | 0.93111 |
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Target: 5'- -cGGaGCAcgUgAUUGAaaGCCGUCGUCGCg -3' miRNA: 3'- cuCC-CGU--AgUAACUa-CGGCAGCGGUG- -5' |
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| 16581 | 5' | -55 | NC_004117.1 | + | 119912 | 0.66 | 0.925776 |
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Target: 5'- cGGGGGCAU---UGGUGCCuagUGCCGa -3' miRNA: 3'- -CUCCCGUAguaACUACGGca-GCGGUg -5' |
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| 16581 | 5' | -55 | NC_004117.1 | + | 132644 | 0.67 | 0.881601 |
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Target: 5'- uAGGGCAgcUAUUGuUGCCGUCGUg-- -3' miRNA: 3'- cUCCCGUa-GUAACuACGGCAGCGgug -5' |
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| 16581 | 5' | -55 | NC_004117.1 | + | 111380 | 0.67 | 0.874344 |
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Target: 5'- -cGuGCGaCAguUUGAUGCCGUCGgCCGCa -3' miRNA: 3'- cuCcCGUaGU--AACUACGGCAGC-GGUG- -5' |
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| 16581 | 5' | -55 | NC_004117.1 | + | 99456 | 0.68 | 0.866864 |
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Target: 5'- -cGGGCAcgguuauguUCAcuuUUGGUGCUGUCGCgGa -3' miRNA: 3'- cuCCCGU---------AGU---AACUACGGCAGCGgUg -5' |
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| 16581 | 5' | -55 | NC_004117.1 | + | 30924 | 0.72 | 0.619687 |
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Target: 5'- cGAcGGCAUaggauuGUUGAUGCCGaCGCCGCc -3' miRNA: 3'- -CUcCCGUAg-----UAACUACGGCaGCGGUG- -5' |
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| 16581 | 5' | -55 | NC_004117.1 | + | 19750 | 1.08 | 0.003575 |
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Target: 5'- uGAGGGCAUCAUUGAUGCCGUCGCCACc -3' miRNA: 3'- -CUCCCGUAGUAACUACGGCAGCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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